PGI-RO

Phosphoglucose Isomerase (PGI)

from yeast

• Synonym(s): glucose-6-phosphate isomerase
• UNSPSC Code: 12352204
• EC Number: 5.3.1.9 (BRENDA, IUBMB)
• NACRES: NA.54

Properties

• Product Name: Phosphoglucose Isomerase (PGI), from yeast
• biological source: yeast
• form: suspension
• specific activity: ~350 units/mg protein (at 25 °C with fructose-6-P as the substrate.)
• packaging: pkg of 1 mL (10127396001 [2 mg]); pkg of 1 mL (10128139001 [10 mg])
• manufacturer/tradename: Roche
• optimum pH: 7.0-7.6
• storage temp.: 2-8°C
• Quality Level: 100

Description

Analysis Note

Contaminants: <0.01% F6P-K, GR, 6-PGDH and PGluM, each, <0.2% β-fructosidase

Application

Isomerization of ketoses to aldoses.

Biochem/physiol Actions

Phosphoglucose isomerase (PGI) is specific for G6P (K_m = 0.7 mM) and fructose-6-phosphate (F6P).

General description

Phosphoglucose Isomerase is an enzyme which possesses diagnostic potential when fractionated by cellulose acetate electrophoresis. This cytosolic enzyme plays an important role during glycolysis and glucogenesis. It acts as an tumor-secreted cytokine and autocrine motility factor. It is used as neurotrophic factor, maturation factor and as serine proteinase inhibitor.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Physical form

Suspension in 3.2 M ammonium sulfate solution, pH approximately 6

Preparation Note

Storage conditions (working solution): A solution of PGI in 20 mM Tris, pH 8.1, containing 0.2 mg BSA/ml, is stable approx. 1 week at 2 to 8 °C.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: No data available
• flash_point_c: No data available