GPT-RO

Glutamate-Pyruvate Transaminase (GPT)

from pig heart

• Synonym(s): alanine aminotransferase
• UNSPSC Code: 12352204
• EC Number: 2.6.1.2 (BRENDA, IUBMB)
• Specific activity: ≥80 units/mg protein (At 25 °C with L-alanine and 2-oxoglutarate as the substrates.)
• Biological source: pig heart

Properties

• biological source: pig heart
• form: suspension
• specific activity: ≥80 units/mg protein (At 25 °C with L-alanine and 2-oxoglutarate as the substrates.)
• packaging: pkg of 1 mL (10105589001 [10 mg]), pkg of 2.5 mL (10737127001 [25 mg])
• manufacturer/tradename: Roche
• optimum pH: ~8.0
• shipped in: wet ice
• storage temp.: 2-8°C

Description

General description

Glutamate-pyruvate transaminase is a blood enzyme. It metabolizes glutamate in blood resulting in low extracellular levels of brain glutamate.

Application

Synthesis of unnatural L-amino acids from α-keto acids.

Physical form

Suspension in 3.2 M ammonium sulfate solution, pH approximately 6

Preparation Note

Activator: Pyridoxalphosphate
Storage conditions (working solution): The enzyme is stable when dissolved in human serum and frozen in aliquots. For best results, centrifuge the enzyme and decant the supernatant before dissolving the enzyme in human serum to avoid high ammonium sulfate concentrations in the solution.

Analysis Note

Contaminants: < 0.01% GIDH, LDH, and MDH each, < 0.03% GOT

Other Notes

For life science research only. Not for use in diagnostic procedures.

Unit Conversion: 1 U [+25 °C, pH 7.5] ≈ 1.75 U [+37 °C, pH 7.5]

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: No data available
• flash_point_c: No data available