The exact concentration of Wnt3a in this product is considered proprietary and may not be disclosed.
SCM112
3dGRO® Wnt3a Conditioned Media Supplement
Alternative stem cell media supplement to purified recombinant Wnt3a protein for organoid cultures
Synonym(s):
Wnt3a Conditioned Media;Wnt3a CM;Wnt Conditioned Media, Wnt3a
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About This Item
NACRES:
NA.76
UNSPSC Code:
41106514
Pricing and availability is not currently available.
sterility
sterile (No Growth/Pass)
form
liquid
packaging
1 ea of 50 mL
manufacturer/tradename
Millipore
technique(s)
cell culture | mammalian: suitable, cell culture | stem cell: suitable
shipped in
dry ice
storage temp.
−20°C
Application
- Appearance (color): clear, red liquid
- Endotoxin: 2 EU/mL
- Sterility Tested: No Growth/Pass
- Mycoplasma: Negative
- Media tested for function in supporting growth of human pancreatic organoids
- Media tested for biological activity using a Wnt3a luciferase reporter assay in HEK293 STF cells
Biochem/physiol Actions
3D organoid culture systems are increasingly employed as powerful tools for the study of human diseases. Wnt proteins hetero-dimerize Frizzled (Fzd) family receptors and their co-receptors Lrp5/6, triggering downstream signaling pathways including the canonical β-catenin resulting in expression of genes regulating cell development. Wnt3a is considered an essential protein in many organoid culture systems (intestinal, pancreatic, liver, colon etc.) because the proliferation of stem cells is governed by the Wnt signaling pathway. However, due to the high concentration requirement in organoid media, the use of the recombinant Wnt3a becomes prohibitively expensive. Additionally, recombinant Wnt3a proteins are often unstable and lack biological activity. The 3dGRO® Wnt3a Conditioned Media Supplement is a potent and inexpensive alternative to purified recombinant Wnt3a protein for organoid cultures and is optimized for the highest level of Wnt3a expression to ensure consistency and performance.SourceThe 3dGRO® Wnt3a Conditioned Media Supplement is generated from mouse L-M(TK-) cells transfected with a mouse Wnt-3A expression vector. The conditioned medium contains fetal bovine serum.
Features and Benefits
Alternative stem cell media supplement to purified recombinant Wnt3a protein for organoid cultures.
Preparation Note
Wnt3a Conditioned Medium should be stored at -20°C. When ready to use, thaw the supplement overnight at 2-8°C in the dark. Thawed Wnt3a Conditioned Medium is good for two weeks when stored at 2-8°C.
Legal Information
3dGRO is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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This Item | |||
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| description Alternative stem cell media supplement to purified recombinant Wnt3a protein for organoid cultures | description Complete serum-free organoid media for the expansion and long-term 3D culture of human intestinal colon organoids. | description ReNcell NSC Maintenance Medium is a defined serum-free, growth factor-free neural stem cell medium that has been optimized for the growth & in vitro differentiation of ReNcell immortalized human neural progenitor cells. | description Advanced DMEMF12 basal media used in low-serum or serum-free media formulations including stem cell and 3D organoid cell cultures. |
| technique(s) cell culture | stem cell: suitable, cell culture | mammalian: suitable | technique(s) cell culture | stem cell: suitable | technique(s) cell culture | stem cell: suitable | technique(s) cell culture | stem cell: suitable |
| sterility sterile (No Growth/Pass) | sterility - | sterility - | sterility - |
| form liquid | form liquid | form liquid | form liquid |
| storage temp. −20°C | storage temp. - | storage temp. -10 to -25°C | storage temp. - |
| packaging 1 ea of 50 mL | packaging pkg of 50 mL | packaging - | packaging - |
| manufacturer/tradename Millipore | manufacturer/tradename - | manufacturer/tradename Chemicon® | manufacturer/tradename - |
Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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What's the concentration of Wnt3a in this medium?
1 answer-
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What's the percentage of FBS contained in SCM112? Thanks!
1 answer-
The base medium of SCM112 is DMEM with FBS (cat# D5796). This DMEM includes 0.2g/L CaCl2. The exact percentage is proprietary.
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