681665

Wnt Agonist

The Wnt Agonist, also referenced under CAS 853220-52-7, controls the biological activity of Wnt. This small molecule/inhibitor is primarily used for Cancer applications.

• Synonym(s): Wnt Agonist, 2-Amino-4-(3,4-(methylenedioxy)benzylamino)-6-(3-methoxyphenyl)pyrimidine, Wnt Pathway Activator I
• Empirical Formula (Hill Notation): C₁₉H₁₈N₄O₃
• CAS Number: 853220-52-7
• Molecular Weight: 350.37
• MDL number: MFCD09908087
• UNSPSC Code: 12352200
• NACRES: NA.77
• Assay: ≥95% (HPLC)
• Form: solid (sticky)
• Storage condition: OK to freeze, protect from light

Properties

• Quality Segment: 100
• assay: ≥95% (HPLC)
• form: solid (sticky)
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, protect from light
• color: yellowish
• solubility: DMSO: 10 mg/mL, ethanol: 5 mg/mL
• shipped in: ambient
• storage temp.: 2-8°C
• SMILES string: N(Cc3cc4c(cc3)OCO4)c1nc(nc(c1)c2cc(ccc2)OC)N
• InChI: 1S/C19H18N4O3/c1-24-14-4-2-3-13(8-14)15-9-18(23-19(20)22-15)21-10-12-5-6-16-17(7-12)26-11-25-16/h2-9H,10-11H2,1H3,(H3,20,21,22,23)
• InChI key: FABQUVYDAXWUQP-UHFFFAOYSA-N

Description

General description

A cell-permeable, potent, and selective activator of Wnt signaling that does not inhibit the activity of GSK-3β (IC₅₀ >60 µM). Shown to mimic the effect of Wnt and induce β-catenin and TCF (T-cell fate)-dependent transcriptional activity (EC₅₀ = 700 nM in HEK-293T cells). Reported to cause substantial head defects during Xenopus embryonic development.

Biochem/physiol Actions

Cell permeable: yes

EC50 = 700 nM inducing β-catenin and TCF (T-cell fate)-dependent transcriptional activity in HEK-293T cells

Primary Target
Selective activator of Wnt signaling

Product does not compete with ATP.

Reversible: no

Packaging

Packaged under inert gas

Preparation Note

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Other Notes

Liu, J., et al. 2005. Angew. Chem. Int. Ed.44, 1987.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable