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Trypsin, Porcine, MS Grade

Name: Trypsin, Porcine, MS Grade
Brand: MM

Synonym(s):

Porcine Trypsin

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About This Item

NACRES:

NA.54

UNSPSC Code:

12352202

Specific activity:

≥10,000 U/mg

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Properties

grade

Proteomics Grade

Quality Segment

100

form

lyophilized

specific activity

≥10,000 U/mg

manufacturer/tradename

Calbiochem^®

storage condition

OK to freeze

suitability

suitable for mass spectrometry

foreign activity

Chymostrypsin <3 BTEE units/mg solid

shipped in

wet ice

storage temp.

−20°C

Description

General description

A chemically modified trypsin that is resistant to autolysis and degradation. Trypsin specifically hydrolyzes amides, peptides and proteins at the C-terminus side of arginine and lysine residues. This mass spectrometry grade trypsin is methylated, TPCK treated, and affinity purified. It is specifically designed for digestion of proteins for sequencing and peptide fragment analysis by mass spectrometry. A trypsin to protein ratio of 1:100 to 1:20 is recommended for mass spectrometry protocols. Note: 1 SET = 5 X 20 µg of modified trypsin, and 1 ml of resuspension buffer.

Preparation Note

Following reconstitution, aliquot and freeze (-°C). Unused stock solutions are stable for up to 2 weeks at -70°C. Avoid freeze/thaw cycles of solution.

Other Notes

Henzel, W.J., et al. 1993. PNAS90, 5011.
Rice, R.H., et al. 1977. Biochim Biophys Acta492, 316..

One BAEE unit is defined as the ammount of enzyme required to produce a ΔA253 of 0.001 per min in a reaction volume of 3.2 ml at pH 7.6 at 25°C.

One BTEE unit is defined as the amount of enzyme required to hydrolyze 1 µmol BTEE per min at 25°C, pH 7.8

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Harmful (C)

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1 of 1

This Item	1700013	T6567	T0303
Sigma-Aldrich 650279 Trypsin, Porcine, MS Grade	USP 1700013 Trypsin Recombinant Porcine	Sigma-Aldrich T6567 Trypsin from porcine pancreas	Sigma-Aldrich T0303 Trypsin from porcine pancreas
description -	description United States Pharmacopeia (USP) Reference Standard	description BioReagent, Proteomics Grade, Dimethylated	description Type IX-S, lyophilized powder, 13,000-20,000 BAEE units/mg protein
specific activity ≥10,000 U/mg	specific activity -	specific activity -	specific activity 13,000-20,000 BAEE units/mg protein
grade Proteomics Grade	grade pharmaceutical primary standard	grade -	grade -
form lyophilized	form liquid	form -	form lyophilized powder
suitability suitable for mass spectrometry	suitability -	suitability -	suitability -
storage condition OK to freeze	storage condition -	storage condition -	storage condition -
shipped in wet ice	shipped in -	shipped in wet ice	shipped in -

Pictograms

GHS08,GHS07

Signal Word

Danger

Hazard Codes

H315,H319,H334,H335

Precautionary Statements

P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class

10 - Combustible liquids

WGK

WGK 1

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