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126741

Alcalase® Enzyme, Bacillus licheniformis

Synonym(s):

Protease from Bacillus licheniformis, subtilisin A

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About This Item

EC Number:
NACRES:
NA.56
UNSPSC Code:
12352202
Specific activity:
≥2.4  U/g
Biological source:
Bacillus licheniformis
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biological source

Bacillus licheniformis

Quality Segment

form

liquid

specific activity

≥2.4  U/g

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

technique(s)

activity assay: suitable

color

dark brown

application(s)

life science and biopharma

shipped in

ambient

storage temp.

2-8°C

General description

Alcalase® is a serine endopeptidase that consists primarily of subtilisin A.

Application

Alcalase® Enzyme, Bacillus licheniformis has been used in the proteolytic digestion and extraction of polysaccharides in various sponge species. It has also been used as an endoprotease enzyme in enzymatic hydrolysis for the production of fish protein hydrolysate.

Biochem/physiol Actions

Alcalase® is suitable for the hydrolysis of proteins with an optimal pH of 7.0-8.5. It is used as an additive in detergent, dehairing, and bating leather. Alcalase is also used in meat tenderizing, cheese flavor development, baked manufacture, and improving the digestibility of animal feeds. Commercial Alcalase® comprises a liquid enzymatic preparation containing 50% glycerol, 41% water, and 9% protease extract from Bacillus licheniformis.

Preparation Note

Please refer to the vial label for lot-specific density.

Other Notes

Activity expressed in Anson Units.

Legal Information

Alcalase is a registered trademark of Novozymes Corp.
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Harmful (C)

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This Item
P4860P5380A3403
description

-

description

≥2.4 U/g

description

Type VIII, lyophilized powder, 7-15 units/mg solid

description

Type XII-A, saline solution, ≥500 units/mg protein (biuret)

biological source

Bacillus licheniformis

biological source

-

biological source

Bacillus licheniformis

biological source

-

specific activity

≥2.4  U/g

specific activity

≥2.4 U/g

specific activity

7-15 units/mg solid

specific activity

≥500 units/mg protein (biuret)

technique(s)

activity assay: suitable

technique(s)

-

technique(s)

-

technique(s)

-

application(s)

life science and biopharma

application(s)

-

application(s)

-

application(s)

-

form

liquid

form

aqueous solution

form

lyophilized powder

form

saline solution

storage condition

OK to freeze

storage condition

-

storage condition

-

storage condition

-


Pictograms

Health hazardCorrosion

Signal Word

Danger

Hazard Codes

Hazard Classifications

Aquatic Chronic 3 - Eye Dam. 1 - Resp. Sens. 1

Storage Class

10 - Combustible liquids

WGK

WGK 2

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable



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Questions

1–2 of 2 Questions  
  1. What is the density or the concentration of the enzyme because I can't find it in the FDS ?

    1 answer
    1. Product density and activity are reported in the batch-specific Certificate of Analysis (CofA). The specifications are as follows: density ≥ 1.000 g/ml and activity ≥ 0.750 U/ml.

      Helpful?

  2. Can I dilute the enzyme in distilled/deionized water or should I use any specific buffer for dilution?

    1 answer
    1. Double distilled water can be used to further dilute product 126741.

      Helpful?

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