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Urea

Name: Urea
Brand: MM

for biochemistry

Synonym(s):

Carbamide, Carbonyl diamide, Diaminomethanone, Carbonyldiamine, Carbamide, Carbonyldiamide

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About This Item

Linear Formula:

NH₂CONH₂

CAS Number:

57-13-6

Molecular Weight:

60.06

UNSPSC Code:

12352111

NACRES:

NA.21

EC Index Number:

200-315-5

MDL number:

MFCD00008022

Beilstein/REAXYS Number:

635724

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Properties

vapor pressure

<0.1 hPa ( 20 °C)

Quality Segment

100

form

solid

potency

8471 mg/kg LD₅₀, oral (Rat), 8200 mg/kg LD₅₀, skin (Rat)

technique(s)

titration: suitable

pH

9 (20 °C, 100 g/L in H₂O)

mp

132-135 °C (lit.)

solubility

1000 g/L

density

1.335 g/mL at 25 °C (lit.)

bulk density

720‑760 kg/m³

storage temp.

10-30°C

SMILES string

NC(N)=O

InChI

1S/CH4N2O/c2-1(3)4/h(H4,2,3,4)

InChI key

XSQUKJJJFZCRTK-UHFFFAOYSA-N

Description

Application

Used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form.

Analysis Note

Purity (DSC (differential scanning calorimetry)): ≥ 99.5 %
Identity (IR-spectrum): passes test
Appearance: Colorless crystals or white crystalline powder.
Appearance of solution (100 g/l, water): Clear and colorless.
Melting point (DSC (differential scanning calorimetry)): 132 - 135 °C
UV-Absorption (260 nm; 8 mol/l; 1 cm; water): ≤ 0.1
UV-Absorption (280 nm; 8 mol/l; 1 cm; water): ≤ 0.1
Cyanide (CN): ≤ 0.000001 %
Chloride (Cl): ≤ 0.0005 %
Sulfate (SO₄): ≤ 0.001 %
Heavy metals (as Pb): ≤ 0.0004 %
Fe (Iron): ≤ 0.00002 %
NH₄ (Ammonium): ≤ 0.00001 %
Biuret: ≤ 0.05 %
Sulfated ash (800 °C): ≤ 0.01 %

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This Item	51456	818710	U5378
Millipore 108488 Urea	Sigma-Aldrich 51456 Urea	Sigma-Aldrich 818710 Urea	Sigma-Aldrich U5378 Urea
description for biochemistry	description BioUltra, Molecular Biology, 99% (T)	description for synthesis	description powder, BioReagent, Molecular Biology, suitable for cell culture
technique(s) titration: suitable	technique(s) -	technique(s) -	technique(s) cell culture \| mammalian: suitable
mp 132-135 °C (lit.)	mp 132-135 °C (lit.)	mp 132-135 °C (lit.)	mp 132-135 °C (lit.)
Quality Level 100	Quality Level 200	Quality Level 200	Quality Level 200
bulk density 720‑760 kg/m³	bulk density -	bulk density 720‑760 kg/m³	bulk density -
potency 8471 mg/kg LD₅₀, oral (Rat), 8200 mg/kg LD₅₀, skin (Rat)	potency -	potency 8471 mg/kg LD₅₀, oral (Rat), 8200 mg/kg LD₅₀, skin (Rat)	potency -
solubility 1000 g/L	solubility H₂O: 8 M at 20 °C, clear, colorless	solubility 1000 g/L	solubility H₂O: soluble 480 mg/mL, clear, colorless (8M)

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Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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