Cellulase from Aspergillus niger catalyzes the hydrolysis of endo-1,4-β-D-glycosidic linkages in cellulose, lichenin, barley glucan, and the cellooligosaccharides cellotriose to cellohexaose. It does not cleave cellobiose or p-nitrophenyl-β-D-glucoside. This enzyme will also cleave intact glycosaminoglycan from a core peptide by hydrolyzing the xylosyl serine linkage.
To screen, identify bacterial strains with high capability to degrade cellulose from bacteria associated with Bursaphelenchus xylophilus and to clone related genes. First, we collected B. xylophilus samples from pine wood nematode disease areas in Nanyang, Henan province, China. Then
Several aminopeptidases of the M42 family have been described as tetrahedral-shaped dodecameric (TET) aminopeptidases. A current hypothesis suggests that these enzymes are involved, along with the tricorn peptidase, in degrading peptides produced by the proteasome. Yet the M42 family remains
Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials, 35(7), 1144-1147 (2012-12-21)
To study the optimum extraction process of total flavonoids from Psoralea coryl folia by cellulose-assisted technique. Based on single-factor experiments, the effects of pH value, temperature of enzymatic hydrolysis, time of enzymatic hydrolysis and enzyme amount on the extraction yields
The Journal of biological chemistry, 288(8), 5861-5872 (2013-01-11)
Root rot fungi of the Heterobasidion annosum complex are the most damaging pathogens in temperate forests, and the recently sequenced Heterobasidion irregulare genome revealed over 280 carbohydrate-active enzymes. Here, H. irregulare was grown on biomass, and the most abundant protein
To isolate and identify the cellulose-producing bacterium from fresh feces of healthy giant pandas, and characterize its cellulase production. A strain with high activity of cellulase was isolated and purified by carboxymethyl cellulose-Na (CMC-Na) medium. The morphological, physiological and biochemical
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