ZRB2186

Anti-Myosin-10 Antibody, clone 1B3 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): Myosin-10;Cellular myosin heavy chain type B;Myosin heavy chain 10;Myosin heavy chain non-muscle IIb; Non-muscle myosin heavy chain B;NMMHC-B:Non-muscle myosin heavy chain IIb:NMMHC II-b;NMMHC-IIB
• UNSPSC Code: 12352203
• NACRES: NA.43

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 1B3, recombinant monoclonal
• description: 1B3 Clone
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 229 kDa; observed mol wt ~230 kDa
• purified by: using Protein A
• species reactivity: rat, mouse, monkey, human
• packaging: antibody small pack of 25 μL
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• technique(s): affinity binding assay: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgG
• epitope sequence: C-terminal
• Protein ID accession no.: NP_005955
• UniProt accession no.: P35580
• greener alternative category: , Aligned
• shipped in: ambient
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... MYH10(4628)

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone B3 is a ZooMAb^® Rabbit recombinant monoclonal antibody that specifically detects Myosin-10. It targets an epitope within 24 amino acids from the C-terminal region.

Immunogen

KLH-conjugated linear peptide corresponding to 24 amino acids from the C-terminal region of human Myosin-10.

Application

Quality Control Testing

Evaluated by Western Blotting in C6 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Myosin-10 in C6 cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Myosin-10 in lysates from COS-7 and NIH3T3 cells and Human brain tissue and Mouse brain tissue.

Affinity Binding Assay: A representative lot of this antibody bound Myosin-10 peptide with a KD of 9.6 x 10-8 in an affinity binding assay.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Myosin-10 in human cerebellum tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Target description

Myosin-10 (UniProt: P35580; also known as Cellular myosin heavy chain, type B, Myosin heavy chain 10, Myosin heavy chain, non-muscle IIb, Non-muscle myosin heavy chain B, NMMHC-B, Non-muscle myosin heavy chain IIb, NMMHC II-b, NMMHC-IIB) is encoded by the MYH10 gene (Gene ID: 4628) in human. Myosins are highly conserved, ubiquitously expressed proteins that form regular bipolar thick filaments that together with actin thin filaments constitute the fundamental contractile unit of skeletal and cardiac muscle. Myosin-10 is a member of the MyTH4/FERM domain family of unconventional, actin-based motor proteins. It contains two heavy chain subunits (MHC), two alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2). It has a rodlike tail sequence that is highly repetitive, showing cycles of a 28-residue repeat pattern composed of four heptapeptides, characteristic for -helical coiled coils. It is mainly localized to cell projections and lamellipodia. Myosin-10 plays a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. During cell spreading, it plays an important role in cytoskeleton reorganization, focal contacts formation, and lamellipodial extension. Myosin-10 is reported to drive glioblastoma growth by stimulating integrin-dependent signaling. Its levels are upregulated in lung, prostate, and breast carcinomas. Myosin-10 knockout mice can survive to adulthood, implying that normal cells can compensate for its loss. Its deletion is shown to impair invasion, slow proliferation, and prolongs survival in murine models of glioblastoma. Five isoforms of Myosin-10 have been described that are produced by alternative splicing. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Kenchappa, RS., et al. (2020). iScience. 23(12); 101802; Betapudi, V. (2010). PLoS One 5(1); e8560).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable