The concentration for product T6025 is 40 mM Tris acetate with 1 mM EDTA at approximately pH 8.3. This solution, prepared with 18 megohm water and 0.2 μm filtration, is considered a 1X working solution.
T6025
Tris Acetate-EDTA buffer
BioReagent, suitable for electrophoresis
Synonym(s):
TAE buffer
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1 L
610,00 kr.
About This Item
Recommended Products
sterility
0.2 μm filtered
Quality Level
product line
BioReagent
form
working solution
technique(s)
electrophoresis: suitable
impurities
DNase, RNase, Protease, none detected
suitability
suitable for electrophoresis
suitable for gel electrophoresis (after dilution to working concentration)
application(s)
diagnostic assay manufacturing
SMILES string
CC(O)=O.NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O
InChI
1S/C10H16N2O8.C4H11NO3.C2H4O2/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8;1-2(3)4/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2;1H3,(H,3,4)
InChI key
HGEVZDLYZYVYHD-UHFFFAOYSA-N
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Application
TAE running buffer is the most commonly used buffer for DNA agarose gel electrophoresis but is also used for non-denaturing RNA agarose gel electrophoresis. Double-stranded DNA tends to run faster in TAE than in other buffers but can also become exhausted during extended electrophoresis. Buffer circulation or buffer replacement during extended electrophoresis can remedy the lower buffering capacity. Dilution of the concentrated TAE buffer produces a 1× TAE buffer with 40 mM Tris-acetate and 1 mM EDTA, pH 8.3. The 1× TAE buffer is used both in the agarose gel and as a running buffer. Applied voltages of less than 5 V/cm (distance between the electrodes of the unit) are recommended for maximum resolution.
Other Notes
40 mM Tris acetate, pH approx. 8.3, containing 1 mM EDTA.
Preparation Note
Prepared with Biotechnology Performance Certified Trizma base (T6066) and Molecular Biology Reagent EDTA disodium salt (E5134). Solutions also contain acetic acid (A6283); powdered blend contains Trizma acetate (T1258).
Solution prepared with 18 megohm water
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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