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T5513

Sigma-Aldrich

N,N,N′,N′-Tetramethylbenzidine

≥95% (HPLC)

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About This Item

Linear Formula:
[-C6H4-4-N(CH3)2]2
CAS Number:
366-29-0
Molecular Weight:
240.34
EC Number:
206-676-5
MDL number:
MFCD00008310
UNSPSC Code:
12352103
PubChem Substance ID:
24900288
NACRES:
NA.32

Assay

≥95% (HPLC)

form

powder

mp

193-195 °C (lit.)

SMILES string

CN(C)c1ccc(cc1)-c2ccc(cc2)N(C)C

InChI

1S/C16H20N2/c1-17(2)15-9-5-13(6-10-15)14-7-11-16(12-8-14)18(3)4/h5-12H,1-4H3

InChI key

YRNWIFYIFSBPAU-UHFFFAOYSA-N

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Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H315,H319,H335

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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C Stürmer et al.
Neuroscience letters, 23(1), 1-5 (1981-04-09)
Tetramethylbenzidine (TMB) histochemistry for retrogradely transported horseradish peroxidase (HRP) has been successfully applied in electron microscopical preparations in the guinea pig visual cortex. In ultrathin sections, the TMB--HRP reaction product appears as an accumulation of typical electron-dense crystals. These crystals
T M Bricker et al.
Biochimica et biophysica acta, 1409(1), 50-57 (1998-11-07)
Site-directed mutagenesis was used to produce a Synechocystis mutant containing a histidine tag at the C terminus of the CP 47 protein of Photosystem II. This mutant cell line, designated HT-3, exhibited slightly above normal rates of oxygen evolution and
L G McGirr et al.
Chemico-biological interactions, 61(1), 61-74 (1987-01-01)
The mechanism of peroxidative N-dealkylation of alkylamines proceeds via one-electron oxidation to the iminium cation which reacts with water to give the N-hydroxymethyl derivative which decomposes to formaldehyde and the N-demethylated product. This reaction is normally inhibited by glutathione by
D I Metelitsa et al.
Biokhimiia (Moscow, Russia), 61(2), 308-321 (1996-02-01)
The tetramethylbenzidine (TMB) oxidation kinetics dependent on spleen ferritin (FERR) has been studied in TMB-FERR-H2O2 (1) and TMB-FERR-O2 (2) systems at pH 4.2 and 6.0. At TMB concentrations below 6 mM, the dependencies of the initial rate for protein oxidation
F H Pujol et al.
Journal of immunoassay, 14(1-2), 21-31 (1993-03-01)
An enzyme immunoassay for detection of hepatitis B surface antigen based on the use of 3 monoclonal antibodies (mAbs) was developed: an IgM as capture and 2 IgG1 for detection. The system biotin-streptavidin was compared with direct conjugation of mAbs
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