SAB4700051

Monoclonal Anti-CD3-PE antibody produced in mouse

clone APA1/1, purified immunoglobulin, buffered aqueous solution

• Synonym(s): Anti-CD3 (activation epitope)
• UNSPSC Code: 12352203
• NACRES: NA.44

Properties

• biological source: mouse
• conjugate: phycoerythrin (R-PE) conjugate
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: APA1/1, monoclonal
• form: buffered aqueous solution
• species reactivity: human, mouse
• concentration: 0.1 mg/mL
• technique(s): flow cytometry: suitable
• isotype: IgG1
• NCBI accession no.: NM_000733.3
• shipped in: wet ice
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... CD3(916)

Description

General description

The mouse monoclonal antibody APA1/1 recognizes an activation-dependent intracellular epitope of CD3 epsilon. Exposure of the epitope precedes CD3 phosphorylation and recruitment and activation of ZAP70, which initiates the signaling cascade produced by T-cell activation. APA1/1 provides the earliest known marker for TCR-mediated T cell activation.

Immunogen

Purified human CD3 proteins isolated from thymus

Application

The reagent is designed for Flow Cytometry analysis. Suggested working dilution is 5 μg/mL of sample. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Physical form

Solution in phosphate buffered saline containing 15 mM sodium azide and 0.2% high-grade protease free BSA as a stabilizing agent.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable