Anti-eukaryotic translation initiation factor 2C 2 (EIF2C2)/protein argonaute-2 (Ago3) is produced in rabbit using as immunogen a synthetic peptide corresponding to an internal region sequence of human EIF2C2/Ago2 conjugated to KLH. Argonaute proteins have a molecular weight of about 100 kDa and are characterized by piwi-argonaute-zwille (PAZ) and PIWI domains. Ago subfamily consists of hsAgo1-4 (also known as EIF2C1-4). Ago proteins localize to the cytoplasm of somatic cells and are concentrated in cytoplasmic processing bodies.
Immunogen
synthetic peptide corresponding to an internal region sequence of human EIF2C2/Ago2, conjugated to KLH. The corresponding sequence is identical in rat and mouse.
Application
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Western Blotting (1 paper)
Biochem/physiol Actions
Ago2 has endonuclease activity, that cleaves the target RNA in RNAi. Ago2 is regulated at both the transcriptional and posttranslational levels in human breast cancer cell lines. It is implicated in micro-RNA activity and in the tumorigenic progression of breast cancer cell lines.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
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Argonaute-2 expression is regulated by epidermal growth factor receptor and mitogen-activated protein kinase signaling and correlates with a transformed phenotype in breast cancer cells
Antibodies targeting receptor-mediated entry of HCV into hepatocytes confer limited therapeutic benefits. Evidence suggests that exosomes can transfer genetic materials between cells; however, their role in HCV infection remains obscure. Here, we show that exosomes isolated from sera of chronic
The Argonaute protein family
Hock J and Gunter M
Genome Biology, 9(2), 210-210 (2008)
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