Skip to Content
Merck
All Photos(1)

Documents

RAB0618

Sigma-Aldrich

Human APOC3 / Apolipoprotein C-III ELISA Kit

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
41116158
NACRES:
NA.32

species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable

input

sample type plasma
sample type cell culture supernatant(s)
sample type serum

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 2.5 pg/mL
standard curve range: 2.44-10000 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... APOC3(345)

Related Categories

General description

The antibody pair provided in this kit recognizes Human Apolipoprotein C3.
ApoC-III is a glycoprotein that has 79 amino acids. It is coded by APOC3 gene. APOC3 is expressed in the liver and intestine. This gene is located on human chromosome 11.

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Biochem/physiol Actions

Apolipoprotein C-III (APOC3) has the ability to prevent lipoprotein lipase (LPL) and hepatic lipase activity. It is considered as a major modulator of triglyceride metabolism.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.
Description
SDS
  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS
  • RABSTOP3ELISA Stop Solution (Item I)SDS
  • RABWASH420X Wash Buffer (Item B)SDS

Pictograms

Corrosion
GHS05

Signal Word

Warning

Hazard Statements

H290

Precautionary Statements

P234 - P390

Hazard Classifications

Met. Corr. 1

Storage Class Code

8A - Combustible corrosive hazardous materials

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Role of ApoCs in lipoprotein metabolism: functional differences between ApoC1, ApoC2, and ApoC3.
Jong MC, et al.
Arteriosclerosis, Thrombosis, and Vascular Biology, 19(3), 472-484 (1999)
A human APOC3 missense variant and monoclonal antibody accelerate apoC-III clearance and lower triglyceride-rich lipoprotein levels.
Khetarpal SA, et al.
Nature Medicine, 23(9), 1086-1086 (2017)
Keishi Hata et al.
Biomedical research (Tokyo, Japan), 41(1), 33-42 (2020-02-25)
We investigated lipid metabolism in PXB-cells, which are human primary hepatocytes isolated from liver-humanized mice, and HepG2 and HuH-7 human hepatoma cell lines. Lipoprotein levels were higher in PXB-cells than in the 2 other cell lines, and PXB-cells mainly released
Åsa Sandin et al.
Scandinavian journal of clinical and laboratory investigation, 80(3), 230-235 (2020-02-29)
Apolipoprotein CIII (apoCIII) is associated with triglyceride (TG)-rich particles like VLDL and exerts an inhibitory effect of lipoprotein lipase. Increased levels are related to cardiovascular diseases and diabetes and therefore apoCIII has been proposed as a useful biomarker. Even if
Targeting APOC3 in the familial chylomicronemia syndrome.
Gaudet D, et al.
The New England Journal of Medicine, 371(23), 2200-2206 (2014)
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service