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To collect the agarose beads, a centrifugation speed of 1000 x g for 5 minutes should be sufficient, using the A2220 batch method as a guide
P0762
Phosphatase, Alkaline−Agarose from calf intestine
ammonium sulfate suspension
Synonym(s):
Alkaline phosphatase-Agarose
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50 UNITS
2.010,00 kr.
100 UNITS
3.480,00 kr.
250 UNITS
7.430,00 kr.
1000 UNITS
23.170,00 kr.
2.010,00 kr.
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50 UNITS
2.010,00 kr.
100 UNITS
3.480,00 kr.
250 UNITS
7.430,00 kr.
1000 UNITS
23.170,00 kr.
About This Item
2.010,00 kr.
Please contact Customer Service for Availability
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biological source
bovine (calf) intestine
Quality Level
form
ammonium sulfate suspension
specific activity
250-1000 units/mL packed gel
matrix
beaded agarose
storage temp.
2-8°C
Related Categories
General description
Alkaline phosphatase from calf intestine is a glycoprotein with a molecular weight of 140,000. [1]
Application
Alkaline phosphatase from calf intestine, CIP, removes phosphate groups from the 5′ ends of DNA which aids in cloning techniques to keep DNA from self-ligation. It can be inhibited by homoarginine. [2]
Calf intestinal alkaline phosphatase (CIAP) has been used in a study with the spectrophotometric method to assess the inhibition effect of dansyl-L-phenylalanine. [3] It has also been shown that (CIAP) is able to detoxify the lipopolysaccharide (LPS)-mediated inflammatory response, which could be important in the treatment of LPS-mediated diseases. [4]
Biochem/physiol Actions
Sigma insoluble enzymes are produced by reacting the conventional "soluble" enzyme with an inert base that results in an insoluble compound retaining the activity of the original enzyme. Applications are almost unlimited. By selecting the "inert" base carefully, a highly active "resin" or "gel" can be produced with which the enzyme reaction can be quickly catalyzed by momentary contact with the substrate in a suitable medium. This can be carried out either in a batch slurry or even through a small column. In either case, the insoluble enzyme does not remain as a contaminant of the reaction mix. It can be readily filtered or centrifuged out of the batch slurry, or remain behind in the column. Many unstable enzymes seem to be much more stable in the insoluble form.
Packaging
Package size based on DEA units
Unit Definition
One unit will hydrolyze 1.0 mmole of p-nitrophenyl phosphate per min at pH9.8 at 37 °C.
Physical form
Suspension in 2.0 M (NH4)2SO4, 1 mM MgCl2, 0.1 mM ZnCl2, pH 7
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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1. What is the recommended speed for spinning down the phosphatase, alkaline-agarose when using this product?
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