Yes, gelation is pH dependent. Adjusting the pH to 5.7 is recommended prior to heating to melt the agar and autoclaving to sterilize. Please see the link below to review the general plant culture medium preparation instructions available in the datasheet for product M5519 (formulation without agar):
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/361/537/m5519pis.pdf
M9274
Murashige and Skoog Basal Medium
suitable for plant cell culture, with sucrose and agar
Synonym(s):
MS Basal Medium
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98,00 kr.
10 L
394,00 kr.
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1 L
98,00 kr.
10 L
394,00 kr.
About This Item
Recommended Products
Quality Level
form
powder
technique(s)
cell culture | plant: suitable
application(s)
agriculture
shipped in
ambient
storage temp.
2-8°C
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Application
Formula variant
With the macro- and micronutrients, vitamins, sucrose and agar as described by Murashige and Skoog (1962).
Media Formulation
Media Formulation
Quantity
Formulated to contain 42.4 grams of powder per liter of medium.
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Product No.
Description
Pricing
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Ox. Sol. 3
Storage Class Code
5.1B - Oxidizing hazardous materials
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Listings
Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.
EU REACH SVHC Candidate List
CAS No.
EU REACH Annex XVII (Restriction List)
CAS No.
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Would an incorrect pH level affect the setting of the agar?
1 answer-
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How firm is the gel in the final sterile medium preparation of Murashige and Skoog Basal Medium, Product M9274?
1 answer-
This formulation contains 8g/L of standard agar resulting in fairly firm and rigid culture medium.
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What is the stability of the prepared Murashige and Skoog Basal Medium, Product M9274?
1 answer-
This agar-containing medium can be stored for 3 to 6 months, depending on the stability of any additional supplements, if stored properly with no observable dehydration of the gel. If the medium appears to darken or pull away from the sides of the culture vessel, it should be discarded.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
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How is Murashige and Skoog Basal Medium, Product M9274 different from Product M5519?
1 answer-
M9274 contains sucrose at 30 g/L and agar at 8 g/L; M5519 does not.
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