Human cells to be used for FACS staining may be Fc-blocked by treatment with 1 μg of human IgG per 100,000 cells, for 15 minutes at room temperature. It is not necessary to wash to remove excess IgG before proceeding with staining.
I4506
IgG from human serum
reagent grade, ≥95% (SDS-PAGE), essentially salt-free, lyophilized powder
Synonym(s):
Human IgG
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10 MG
1.240,00 kr.
100 MG
3.530,00 kr.
50 MG
3.980,00 kr.
500 MG
13.530,00 kr.
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10 MG
1.240,00 kr.
100 MG
3.530,00 kr.
50 MG
3.980,00 kr.
500 MG
13.530,00 kr.
About This Item
Recommended Products
conjugate
unconjugated
Quality Level
grade
reagent grade
Assay
≥95% (SDS-PAGE)
form
essentially salt-free, lyophilized powder
composition
Protein, ≥90%
technique(s)
ELISA: suitable
dot immunobinding: suitable
immunoelectrophoresis: suitable
western blot: suitable
storage temp.
2-8°C
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General description
Purified Human IgG is isolated from pooled normal human serum by fractionation and ion-exchange chromatography. The product is supplied as an essentially salt-free (less than 1% sodium), lyophilized powder.
Application
IgG from human serum was used as a standard at a concentration of 1.274 mg/ml in ELISA to determine antibody responses to Porphyromonas gingivalis and Prevotella intermedia.[1] It was used for immunolabeling of Vero, BHK and PtK2 cells at a concentration of 0.2 mg/ml.[2] IgG from human serum was used as a standard in SDS-PAGE of anti-Shigella LPS antibodies.[3]
Biochem/physiol Actions
IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections.[4]
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Product No.
Description
Pricing
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Access high-quality reagents for antibody workflows, including adjuvants, blocking reagents, conjugation kits, and more.
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How can I use Product I4506, IgG from human serum, to block Fc receptors on the cell surface for flow cytometry analysis of cells?
1 answer-
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Is Product I4506, IgG from human serum, from a single donor?
1 answer-
No. Product No. I4506 is from pooled normal serum.
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Is Product I4506, IgG from human serum, tested for endotoxin?
1 answer-
No. Product No. I4506 is not tested for endotoxin levels.
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What isotype is present in Product I4506, IgG from human serum?
1 answer-
Product No. I4506 is IgG, not a myeloma protein. It contains all the IgG isotypes.
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