HPA018157
Anti-PAFAH2 antibody produced in rabbit
Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
Synonym(s):
Anti-HSD-PLA2, Anti-Platelet-activating factor acetylhydrolase 2, cytoplasmic, Anti-Serine-dependent phospholipase A2
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100 μL
4.340,00 kr.
4.340,00 kr.
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100 μL
4.340,00 kr.
About This Item
4.340,00 kr.
Please contact Customer Service for Availability
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
product line
Prestige Antibodies® Powered by Atlas Antibodies
form
buffered aqueous glycerol solution
species reactivity
human
enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
technique(s)
immunoblotting: 0.04-0.4 μg/mL
immunohistochemistry: 1:20-1:50
immunogen sequence
RGFVVAVPEHRDRSAATTYFCKQAPEENQPTNESLQEEWIPFRRVEEGEKEFHVRNPQVHQRVSECLRVLKILQEVTAGQTVFNILPGGLDLMTLKGNIDMSRVAVMGHS
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... PAFAH2(5051)
General description
Platelet-activating factor acetylhydrolases (PAFAHs) are a family of specialized phospholipase A2 enzymes. PAFAH2 is a 40kDa monomer with most abundant expression in bovine liver and kidney. In resting conditions the protein is localized in the cytoplasm as homodimers, and it responds to oxidative stress by binding to endoplasmic reticulum and Golgi membranes as monomers.
Immunogen
Platelet-activating factor acetylhydrolase 2, cytoplasmic recombinant protein epitope signature tag (PrEST)
Application
Anti-PAFAH2 antibody produced in rabbit, a Prestige Antibody, is developed and validated by the Human Protein Atlas (HPA) project . Each antibody is tested by immunohistochemistry against hundreds of normal and disease tissues. These images can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. The antibodies are also tested using immunofluorescence and western blotting. To view these protocols and other useful information about Prestige Antibodies and the HPA, visit sigma.com/prestige.
Biochem/physiol Actions
Platelet-activating factor acetylhydrolases2 (PAFAH2) hydrolyzes platelet-activating factor and oxidatively fragmented phospholipids at the sn-2 position, protecting cells from oxidative stress induced apoptosis. PAFAH2 is up-regulated in patients with extensive coronary artery disease. Overexpression of PAFAH2 protects human keratinocyte-derived cells against apoptosis induced by t-butylhydroperoxide (t-BuOOH) and ultraviolet B radiation. Similarly, overexpression of PAFAH2 in Chinese hamster ovary cells inhibits apoptosis induced by the pro-oxidant t-BuOOH. Presence of PAFAH2 in mice models exerts strong neuroprotective effects against ischemic injury in the central nervous system.
Features and Benefits
Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section.
Every Prestige Antibody is tested in the following ways:
Every Prestige Antibody is tested in the following ways:
- IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues.
- Protein array of 364 human recombinant protein fragments.
Linkage
Corresponding Antigen APREST76147
Physical form
Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide
Legal Information
Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Elizabeth S Monillas et al.
Biochimica et biophysica acta, 1854(5), 469-475 (2015-02-25)
The intracellular enzyme platelet-activating factor acetylhydrolase type-II (PAFAH-II) hydrolyzes platelet-activating factor and oxidatively fragmented phospholipids. PAFAH-II in its resting state is mainly cytoplasmic, and it responds to oxidative stress by becoming increasingly bound to endoplasmic reticulum and Golgi membranes. Numerous
XiaoFei Li et al.
Atherosclerosis, 218(1), 83-89 (2011-05-25)
Inflammation plays a crucial role in plaque vulnerability. Calcifications can be detected by means of in vivo imaging techniques. The study purpose is to assess a potential association between tissue localization of calcifications and the inflammatory biomarkers, C-reactive protein (CRP)
Kimiko Umemura et al.
Stroke, 38(3), 1063-1068 (2007-02-03)
Platelet-activating factor (PAF) and oxidized unsaturated free fatty acids have been postulated to aggravate neuronal damage in the postischemic brain. Type II PAF-acetylhydrolase (PAF-AH II) not only terminates signals by PAF by its PAF-hydrolyzing activity but also protects cells against
Erik Berg Schmidt et al.
Atherosclerosis, 196(1), 420-424 (2006-12-13)
Lipoprotein-associated phospholipase A(2) (Lp-PLA(2)) is an emerging risk factor for cardiovascular disease. In the present study, plasma levels of Lp-PLA(2) were measured in patients (n=301) admitted to elective coronary angiography because of suspected coronary artery disease (CAD). In a multiple
A Matsuzawa et al.
The Journal of biological chemistry, 272(51), 32315-32320 (1998-01-24)
Platelet-activating factor-acetylhydrolase (PAF-AH), which removes the acetyl group at the sn-2 position of PAF, is distributed widely in tissues and plasma. Tissue cytosol contains at least two types of PAF-AH, isoforms Ib and II. Isoform Ib is a tertiary G-protein
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