The use of this antibody for western blotting has been reported in one study on Pseudomonas and tumor cells. See: Leamon, C.P., et al., Cytotoxicity of Folate-Pseudomonas Exotoxin Conjugates Toward Tumor Cells. J. Biol. Chem., 268(33), 24847-24854 (1993). The antibody was used at a 1 to 1000 dilution in Tris buffered saline (TBS) for 1 hour at 23°C.
F5766
Monoclonal Anti-Folic Acid antibody produced in mouse
clone VP-52, ascites fluid
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0.2 ML
4.270,00 kr.
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8.490,00 kr.
About This Item
Recommended Products
biological source
mouse
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
VP-52, monoclonal
contains
15 mM sodium azide
technique(s)
indirect ELISA: 1:500
isotype
IgG2b
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
General description
Monoclonal Anti-Folic Acid (mouse IgG2b isotype) is derived from the hybridoma produced from the fusion of mouse myeloma cells and splenocytes of an immunized mouse. The normal range for folate in serum is 3-13 ng/ml.
Specificity
Monoclonal Anti-Folic Acid antibody binds specifically to folate and detects an epitope present on both folic acid and its biologically active analog 5-methyltetrahydrofolic acid (5MTHFA). The product reacts with free folate, folate conjugated to a carrier such as KLH or BSA, and folic acid in human plasma or serum bound to the endogenous Folate Binder. The antibody does not cross-react with tetrahydrofolic acid, folinic acid or dihydrofolic acid.
Immunogen
5-Methyltetrahydrofolic acid (5MTHFA) conjugated to KLH
Application
Monoclonal Anti-Folic Acid antibody is suitable for use in western blot (diluted 1:1000).
Biochem/physiol Actions
Folic acid (folate) is a vitamin that regulates one-carbon transfer during the conversion of homocysteine to methionine. Methionine is a precursor of S-adenosylmethionine, which is the main methyl donor for methylation reactions. Thus, folic acid is essential for regulation of DNA methylations and for epigenetic modifications that modulate gene expression. Folate deficiency has been associated with a wide range of disorders including colorectal cancers, depression, neural tube defects and cerebral dysfunctions
Folic acid is essential for normal growth of mammalian cells. Folic acid deficiencies are common in pregnant women, alcoholics, those whose diets do not include raw fruits and vegetables, and people with structural or functional damage to the small intestine. Vitamin B12 and folic acid are metabolically interrelated.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Clinical utility of serum folate measurement in tertiary care patients: Argument for revising reference range for serum folate from 3.0 ng/mL to 13.0 ng/mL
Singh G, et al.
practical laboratory medicine, 1, 35-35 (2015)
Linglei Jiang et al.
Biochimica et biophysica acta. General subjects, 1865(4), 129763-129763 (2020-10-17)
Extracellular vesicles (EVs) are nanoparticles which are released by cells from all three domains of life: Archaea, Bacteria and Eukarya. They can mediate cell-cell communication by transferring cargoes such as proteins and nucleic acids between cells. EVs receive great interest
Periconceptional folate deficiency and implications in neural tube defects.
Safi, J., et al.
Journal of Pregnancy, doi: 10-doi: 10 (2012)
Young-In Kim
Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology, 13(4), 511-519 (2004-04-07)
Epidemiological, clinical, and animal studies collectively indicate that dietary folate intake and blood folate levels are inversely associated with colorectal cancer risk. Folate plays an essential role in one-carbon transfer involving remethylation of homocysteine to methionine, which is a precursor
Giuseppe Destito et al.
Chemistry & biology, 14(10), 1152-1162 (2007-10-27)
Cowpea mosaic virus (CPMV) is a well-characterized nanoparticle that has been used for a variety of nanobiotechnology applications. CPMV interacts with several mammalian cell lines and tissues in vivo. To overcome natural CPMV targeting and redirect CPMV particles to cells
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Will Product No. F5766, Monoclonal Anti-Folic Acid antibody, work in Western blotting?
1 answer-
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What is the concentration of Product No. F5766, Monoclonal Anti-Folic Acid antibody?
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The concentration of the antibody is lot specific and can be found on the certificate of analysis. (It is typically in the 1-2 mg/mL range).
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How does the storage temperature relate to shipping conditions?
1 answer-
The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment.
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What immunogen was used to generate Product No. F5766, Monoclonal Anti-Folic Acid antibody?
1 answer-
The immunogen used was 5-Methyltetrahydrofolic acid (sometimes called 5MTH folic acid) conjugated to keyhole limpet hemocyanin (KLH).
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What is the isotype of Product No. F5766, Monoclonal Anti-Folic Acid antibody?
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This antibody is mouse IgG2B.
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What is the name of the antibody clone for Product No. F5766, Monoclonal Anti-Folic Acid antibody?
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The clone is VP-52.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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