E0521
Anti-Estrogen Receptor (18-32) antibody produced in rabbit
IgG fraction of antiserum, buffered aqueous solution
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0.2 ML
3.410,00 kr.
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0.2 ML
3.410,00 kr.
About This Item
biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
IgG fraction of antiserum
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen 67 kDa
species reactivity
human
technique(s)
microarray: suitable
western blot: 1:3000 using MCF7 cultured cell line extract
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... ESR1(2099)
General description
Estrogen is a sex hormone that is a key regulator of growth, differentiation and function of reproductive tissues, skeletal and cardiovascular systems. The effect of estrogen is mediated by two intracellular receptors, ERα and ERβ. Although the two receptors interact with similar ligands, have identical DNA-binding domains and have redundant roles in estrogen signaling, they show distinct expression pattern. On binding to the receptor, the estrogen-ER complex enters nucleus and regulates the transcription of specific genes. The biological effects of estrogen and ER is by upstream activation of the pathway by growth factors. Some pathways that activate the estrogen-ER pathway are those mediated by AP-1, IGF-1, EGF, MAPK and PI3K/Akt . Anti-Estrogen Receptor (18-32) antibody is specific for 18-32 amino acids of human estrogen receptor (67 kDa). The product is an IgG fraction prepared from pooled antisera.
Immunogen
synthetic peptide corresponding to amino acids 18-32 of the human estrogen receptor.
Application
Anti-Estrogen Receptor (18-32) antibody may be used for immunoblotting using extracts of MCF7 cells at a working dilution of 1:3000. It may also be used for dot blot.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Western Blotting (1 paper)
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.2, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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The multifaceted mechanisms of estradiol and estrogen receptor signaling.
J M Hall et al.
The Journal of biological chemistry, 276(40), 36869-36872 (2001-07-19)
Coregulator codes of transcriptional regulation by nuclear receptors.
M G Rosenfeld et al.
The Journal of biological chemistry, 276(40), 36865-36868 (2001-07-19)
J F Couse et al.
Endocrinology, 138(11), 4613-4621 (1997-11-05)
Until recently, only a single type of estrogen receptor (ER) was thought to exist and mediate the genomic effects of the hormone 17beta-estradiol in mammalian tissues. However, the cloning of a gene encoding a second type of ER, termed ERbeta
V Giguère et al.
Nature, 331(6151), 91-94 (1988-01-07)
The gonads and adrenal glands produce steroids classified into five major groups which include the oestrogens, progestins, androgens, glucocorticoids and mineralocorticoids. Gonadal steroids control the differentiation and growth of the reproductive system, induce and maintain sexual characteristics and modulate reproductive
Osman El-Maarri et al.
PloS one, 6(1), e16252-e16252 (2011-02-12)
Previously, we reported on inter-individual and gender specific variations of LINE-1 methylation in healthy individuals. In this study, we investigated whether this variability could be influenced by age or sex hormones in humans. To this end, we studied LINE-1 methylation
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