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D2569

Sigma-Aldrich

Anti-DPPA5 antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-Developmental pluripotency associated 5, Anti-ESG1, Anti-Embryonal stem cell-specific gene 1

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~14 kDa

species reactivity

mouse, human, rat

concentration

~1 mg/mL

technique(s)

immunohistochemistry: 1:100-1:250
western blot: 1:500-1:1,000

UniProt accession no.

A6NC42

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... DPPA5(340168)
mouse ... Dppa5a(434423)
rat ... Dppa5(301101)

General description

The DPPA5 (developmental pluripotency associated 5) protein is strongly expressed in primordial germ cells, and the expression is down-regulated during germ cell development.

Immunogen

synthetic peptide corresponding to residues 43-60 human DPPA5. The sequence is 85% identical in mouse and rat.

Application

Anti-DPPA5 antibody produced in rabbit is suitable for immunohistochemistry at a working dilution of 1:100 to 1:250 and for immunoblotting at 1:500 to 1:1000.

Biochem/physiol Actions

DPPA5 is specifically and differentially expressed in human cells that have pluripotency and can be used as a marker of pluripotent stem cells.

Physical form

solution in phosphate buffered saline, containing 0.02% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Soo-Kyoung Kim et al.
Stem cells (Dayton, Ohio), 23(4), 458-462 (2005-03-26)
Pluripotent embryonic germ cells (EGCs) can be derived from the culture of primordial germ cells (PGCs). However, there are no reports of gonocytes, following the stage of PGC development, becoming stem cell lines. To analyze the gene expression differences between
Ida M A Jentoft et al.
Cell, 186(24), 5308-5327 (2023-11-06)
Mammalian oocytes are filled with poorly understood structures called cytoplasmic lattices. First discovered in the 1960s and speculated to correspond to mammalian yolk, ribosomal arrays, or intermediate filaments, their function has remained enigmatic to date. Here, we show that cytoplasmic
Shichao Liu et al.
Developmental dynamics : an official publication of the American Association of Anatomists, 244(4), 619-627 (2015-01-27)
Mammalian first lineage segregation generates trophectoderm (TE) and pluripotent inner cell mass (ICM), which provides an ideal model for studying the mechanisms of maintenance and loss of pluripotency. In mouse, the transcription factor OCT4 restricts to ICM and plays a

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