CTLL is a subclone of T cells derived from a C57bl/6 mouse. The cells require IL-2 for growth and are used to assay for its presence in conditioned media.
Application
CTLL-2 has been used in CTLL-2 cell proliferation assay to determine the presence of T-cell cytokines.[1][2]
Maintain cultures between 3-9 x 105 cells/ml; 5% CO2; 37°C. Thaw ampoule and perform a cell count, spin out the DMSO and resuspend in an appropriate volume of media to give a cell concentration of 4-5 x 105 cells/ml.
Other Notes
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