Split sub-confluent cultures (70-80%) 1:2 i.e. seeding at 3-5x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37 °C. Passage every 3-4 days.
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ECACC pancreatic cancer cell lines for various applications including drug response studies.
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