B6385
Benzoylated Naphthoylated DEAE–Cellulose
medium
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Application
Benzoylated Naphthoylated DEAE-cellulose is a cellulose medium used in protein chromatography, ion exchange chromatography and anion exchange media. Benzoylated Naphthoylated DEAE is particularly useful for the separation of single and double stranded DNA. Benzoylated Naphthoylated DEAE has been used to design a more efficient approach for the detection of human papillomavirus DNA and to study the cytotoxcity of the drug 5-Fluorouracil (FUra).
Preparation Note
Prepared by the method of Gillam et al., Biochemistry, 6, 3043 (1967).
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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A N Koterov et al.
Biokhimiia (Moscow, Russia), 57(2), 195-200 (1992-02-01)
Single-stranded DNA-binding proteins (SSB-proteins) isolated from Ehrlich ascites tumour (EAT) cells were incubated for 30 min at 5 mM NaCl with salmon sperm DNA or [3H]DNA from EAT at the SSB-protein/DNA ratio (w/w) of 0 to 4.5. After addition of
J D Schuetz et al.
Cancer chemotherapy and pharmacology, 21(3), 208-210 (1988-01-01)
5-Fluorouracil (FUra) was previously demonstrated to be incorporated into DNA at cytotoxic concentrations in mouse bone marrow cells. Subsequently, we showed that under these conditions FUra exhibited a time-dependent removal from DNA accompanied by a decrease in DNA strand length.
R M Knobler et al.
Clinical and experimental dermatology, 17(6), 392-396 (1992-11-01)
Using the benzoylated naphthoylated DEAE cellulose method (BND-method) we have designed a more efficient approach for the detection of human papillomavirus-DNA (HPV-DNA) via dot-blot and hybridization. Biopsy material from anogenital warts (40 patients), invasive carcinoma uteri (12 patients) and normal
Michaela A Gold et al.
PLoS genetics, 17(10), e1009863-e1009863 (2021-10-22)
Disease-associated trinucleotide repeats form secondary DNA structures that interfere with replication and repair. Replication has been implicated as a mechanism that can cause repeat expansions and contractions. However, because structure-forming repeats are also replication barriers, it has been unclear whether
Ana Teixeira-Silva et al.
Nature communications, 8(1), 1982-1982 (2017-12-08)
Replication requires homologous recombination (HR) to stabilize and restart terminally arrested forks. HR-mediated fork processing requires single stranded DNA (ssDNA) gaps and not necessarily double strand breaks. We used genetic and molecular assays to investigate fork-resection and restart at dysfunctional
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