Product No. A8592, ANTI-FLAG M2-peroxidase is supplied as a solution in 10 mM sodium phosphate, pH 7.4, containing 150 mM NaCl and 50% glycerol, plus stabilizer and preservative. The conjugate protein concentration is approximately 1 mg/ml.
A8592
Monoclonal ANTI-FLAG® M2-Peroxidase (HRP) antibody produced in mouse
Monoclonal ANTI-FLAG® M2-Peroxidase (HRP) antibody produced in mouse
clone M2, purified immunoglobulin, buffered aqueous glycerol solution
Synonym(s):
Monoclonal ANTI-FLAG® M2 antibody produced in mouse, Anti-ddddk, Anti-dykddddk
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0.2 MG
5.050,00 kr.
1 MG
9.960,00 kr.
5 X 1 MG
13.450,00 kr.
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0.2 MG
5.050,00 kr.
1 MG
9.960,00 kr.
5 X 1 MG
13.450,00 kr.
About This Item
UNSPSC Code:
41106514
NACRES:
NA.32
clone:
M2, monoclonal
application:
ELISA (i)
species reactivity:
all
technique(s):
indirect ELISA: 1:20,000
citations:
1904
biological source
mouse
Quality Level
conjugate
peroxidase conjugate
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
M2, monoclonal
form
buffered aqueous glycerol solution
species reactivity
all
concentration
~1 mg/mL
technique(s)
indirect ELISA: 1:20,000
isotype
IgG1
immunogen sequence
DYKDDDDK
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... GPX1(2876)
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General description
The Monoclonal ANTI-FLAG M2-Peroxidase is a mouse IgG antibody covalently conjugated to horseradish peroxidase ( HRP). The antibody binds to FLAG fusion proteins and recognizes the FLAG epitope at N-terminal, Met-N-terminal, C-terminal, and internal FLAG peptides.
Application
For simple, one-step detection by immunocytochemistry, ELISA, or Western blotting. Especially useful in detection of FLAG fusion proteins expressed in murine host, where secondary anti-mouse antibodies may cause cross-reactivity.
Suggested dilution for immunocytochemistry and western blotting 1:100 to 1:1000
Suggested dilution for ELISA 1:20,000
Learn more product details in our FLAG® application portal.
Suggested dilution for ELISA 1:20,000
Learn more product details in our FLAG® application portal.
Physical form
Solution in phosphate buffered saline containing 50% glycerol plus preservative and stabilizer
Preparation Note
Dilute ANTI-FLAG M2-Peroxidase solution in Tris Buffered Saline (TBS): 0.05 M Tris, pH 7.4, with 0.15 M NaCl.
Legal Information
ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
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Product No.
Description
Pricing
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Corr. 1C - Skin Sens. 1
Storage Class Code
8A - Combustible corrosive hazardous materials
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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How is Product No. A8592, Monoclonal ANTI-FLAG® M2-Peroxidase (HRP) antibody, supplied?
1 answer-
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What concentration of Product No. A8592, Monoclonal ANTI-FLAG® M2-Peroxidase (HRP) antibody, is recommended for my application?
1 answer-
An antibody titer from 1:100 to 1:1000 is recommended for immunocytochemistry and Western blot. Minimum antibody titer recommended for ELISA is 1:20,000. Adjust antibody titer to maximize detection sensitivity and to minimize background.
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I am getting a high background on my Western blots using Product No. A8592, Monoclonal Anti-FLAG® M2-Peroxidase (HRP) antibody. What can I do to fix this?
1 answer-
There is a troubleshooting guide provided at the end of the product information sheet. Some suggestions include the following: adjusting antibody titer, increase number of washes, increase stringency of washes by addition of 0.05% TWEEN 20 or 0.1% TRITON X-100 to the wash buffers, and/or increase the incubation and wash temperatures.
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What are the appropriate storage conditions for Product No. A8592, Monoclonal ANTI-FLAG® M2-Peroxidase (HRP) antibody?
1 answer-
This product ships on wet ice and it is recommended to store the undiluted antibody at 0 to -20°C. Once diluted, repeated freezing and thawing is not recommended.
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Can product No. A8592, Monoclonal ANTI-FLAG® M2-Peroxidase (HRP) antibody, be used for immunohistochemistry?
1 answer-
The product cannot be used on paraffin embedded tissue sections, but it does work on fixed culture cells (as indicated on the Product Information Sheet) and also may work on frozen sections.
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