71636

Sodium phosphate dibasic

BioUltra, for molecular biology, ≥99.5% (T)

• Synonym(s): sec-Sodium phosphate, Disodium hydrogen phosphate, Disodium phosphate, Sodium hydrogenphosphate
• Linear Formula: Na₂HPO₄
• CAS Number: 7558-79-4
• Molecular Weight: 141.96
• EC Number: 231-448-7
• MDL number: MFCD00003496
• UNSPSC Code: 12161700
• eCl@ss: 38070211
• PubChem Substance ID: 57652328
• NACRES: NA.25

Properties

• grade: for molecular biology
• Quality Level: 200
• product line: BioUltra
• Assay: ≥99.5% (T)
• form: coarse powder; fine crystals; powder
• impurities: DNases, none detected; RNases, none detected; insoluble matter, passes filter test; phosphatases, none detected; proteases, none detected; ≤0.001% total nitrogen (N)
• loss: ≤0.1% loss on drying, 110 °C
• pH: 8.8-9.4 (25 °C, 0.5 M in H₂O)
• pKa (25 °C): (1) 2.15, (2) 6.82, (3) 12.38 (phosphoric acid)
• solubility: H₂O: 0.5 M at 20 °C, clear, colorless
• anion traces: chloride (Cl^-): ≤10 mg/kg; sulfate (SO₄^2-): ≤50 mg/kg
• cation traces: Al: ≤5 mg/kg; As: ≤0.2 mg/kg; Ba: ≤5 mg/kg; Bi: ≤5 mg/kg; Ca: ≤10 mg/kg; Cd: ≤5 mg/kg; Co: ≤5 mg/kg; Cr: ≤5 mg/kg; Cu: ≤5 mg/kg; Fe: ≤5 mg/kg; K: ≤50 mg/kg; Li: ≤5 mg/kg; Mg: ≤5 mg/kg; Mn: ≤5 mg/kg; Mo: ≤5 mg/kg; Ni: ≤5 mg/kg; Pb: ≤5 mg/kg; Sr: ≤10 mg/kg; Zn: ≤5 mg/kg
• λ: 0.5 M in H₂O
• UV absorption: λ: 260 nm A_max: ≤0.025; λ: 280 nm A_max: ≤0.020
• SMILES string: [Na+].[Na+].[H]OP([O-])([O-])=O
• InChI: 1S/2Na.H3O4P/c;;1-5(2,3)4/h;;(H3,1,2,3,4)/q2*+1;/p-2
• InChI key: BNIILDVGGAEEIG-UHFFFAOYSA-L

Description

Application

Sodium phosphate dibasic has been used:

• as a component in phosphate buffer
• in the preparation of MgCl₂ hybridization buffer
• as a component in SSPE solution for the preparation of small RNA hybridisation buffer

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves