SCC279

P493-6 Human Lymphoblastoid Cell Line

• Synonym(s): P-493-6, p493-6
• UNSPSC Code: 41106514
• NACRES: NA.45

Properties

• packaging: vial of ≥1X10⁶ cells/vial
• technique(s): cell culture | mammalian: suitable

Description

General description

The proto-oncogene c-myc is an activator of cell proliferation, promoting cell cycle progression, and is thus significant factor in many cancers. Burkitt’s lymphoma is a rare cancer of human B cells associated with Epstein-Barr virus (EBV) that is the most rapidly growing human tumor, due to transcriptional activation of c-myc by chromosomal translocation (1). Because of its highly aggressive nature, Burkitt’s lymphoma has a high mortality rate if left untreated. Cellular models of c-myc-activated lymphomas are essential for understanding mechanisms of proliferation and for developing treatments for this disease. The P493-6 Human Lymphoblastoid Cell Line expresses c-myc under control of a tetracycline-inducible promoter (2). P493-6 cells constitutively express c-myc when cultured without tetracycline. Addition of tetracycline switches off c-myc expression and arrests cells in G0/G1 phase. P493-6 cells demonstrate the morphology and surface antigen expression typical of Burkitt lymphoma cells, including CD10 and CD38 (3).

Source:
The P493-6 cell line was established from a clone of the EREB2-5 EBV-positive lymphoblastoid cell line stably transfected with a c-myc-tet plasmid construct (2).

References:
1. Lancet. 2012; 379(9822): 1234-1244.
2. Int J Cancer. 2000; 87(6): 787-793.
3. Int J Cancer. 2001; 93(6): 810-816

Cell Line Description

Cancer Cells

Application

• Each vial contains ≥ 1X10⁶ viable cells.
• Cells are tested negative for infectious diseases by a Human Essential CLEAR panel by Charles River Animal Diagnostic Services.
• Cells are verified to be of human origin and negative for inter-species contamination from rat, mouse, chinese hamster, Golden Syrian hamster, and non-human primate (NHP) as assessed by a Contamination CLEAR panel by Charles River Animal Diagnostic Services.
• Cells are negative for mycoplasma contamination.
• Each lot of cells is genotyped by STR analysis to verify the unique identity of the cell line.

Other Notes

This product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact [email protected].

Disclaimer

• This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled.Click here for Enduser Declaration (EUD) Form.
• Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable