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Key Documents

NS03L

Sigma-Aldrich

Normal Mouse Serum

This Normal Mouse Serum is validated for use in ELISA, Flow Cytometry, Immunoblotting, Immunofluorescence, Immunohistochemistry, Immunoprecipitation for the detection of Mouse Serum.

Synonym(s):

Mouse Serum

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1 ML
757,00 kr.

757,00 kr.

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1 ML
757,00 kr.

About This Item

UNSPSC Code:
12352202
NACRES:
NA.43
biological source:
mouse
form:
lyophilized
shipped in:
ambient

757,00 kr.

Please contact Customer Service for Availability
Request a Bulk Order

biological source

mouse

form

lyophilized

contains

≤0.1% sodium azide as preservative

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

shipped in

ambient

storage temp.

2-8°C

General description

Normal carrier sera from healthy, non-immunized mice.

Application

ELISA (see comments)

Flow Cytometry (see comments)

Immunoblotting (see comments)

Immunofluorescence (see comments)

Immunohistochemistry (see comments)

Immunoprecipitation (see comments)

Warning

Toxicity: Standard Handling (A)

Physical form

Lyophilized serum. Lyophilized from 250 mM sodium chloride, 10 mM sodium phosphate, pH 7.6.

Reconstitution

Reconstitute the lypohilized serum in 1 ml of sterile distilled water with occasional gentle mixing for at least 2 h at 4°C. Following reconstitution, aliquot and freeze (-20°C) for long-term storage or refrigerate (4°C) with 0.1% azide for short-term storage. Avoid freeze/thaw cycles of solutions.

Other Notes

Useful as a blocking agent in immunohistochemical procedures or as a negative control for specific mouse antibodies. For use as a negative control in any application, perform experiments in parallel using primary antibody or normal serum at the same concentration. A signal obtained with the normal serum indicates a nonspecific interaction; any additional signal with the primary antibody indicates a specific antibody-antigen interaction. This antibody has also been reported to work for ELISA, flow cytometry, immunoblotting, immunofluorescence, immunohistochemistry, and immunoprecipitation.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Hazard Statements

H412

Precautionary Statements

P273 - P501

Hazard Classifications

Aquatic Chronic 3

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Questions

1–2 of 2 Questions  

  1. Is this product can be used as a replacement of Fc blocker in flow cytometry?

    1 answer

    1. This product is a normal mouse serum and may be used as an Fc blocker in flow cytometry applications. For more information, please see: https://www.sigmaaldrich.com/product/Normal-Mouse-Serum,EMD_BIO-NS03L

      Helpful?

  2. Which strain of mouse is used to derive the serum for product NS03L-1ML?

    1 answer

    1. The serum with catalogue number NS03L-1ML, is pooled from one strain only: Outbred non-swiss white mice, which are also known as Albino mice.

      Helpful?

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