MAB810-M
Anti-Cytomegalovirus Antibody, clone 8B1.2 (ASR)
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About This Item
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antibody form
purified antibody
Quality Level
clone
8B1.2
monoclonal
purified by
using Protein A
species reactivity
human, mouse
concentration
(Please refer to the Certificate of Analysis for the lot-specific concentration.)
technique(s)
immunofluorescence: suitable
isotype
IgG2a
General description
Affinity purified immediate early antigen from MRC-5 cells infected with CMV AD169 (ATCC).
Specificity
Reacts with an immediate early non-structural antigen of 68-72 kDa. This antigen can be detected 1 hour after infection exhibiting an intranuclear staining pattern. This staining reaches a peak at 10-12 hours. This antigen persists and can be detected throughout the complete CMV infection cycle.
Application
ELISA 1:800
Western Blot: 1:200. Recognizes approximately a 70kDa band.
Immunofluorescence: 1:200-1:400.
Immunohistochemistry 1:200-1:800. See general protocols online for guidelines.
Works on paraffin embedded tissue sections.
FACS: See online protocol.
Final working dilutions and protocols must be determined by end user.
Western Blot: 1:200. Recognizes approximately a 70kDa band.
Immunofluorescence: 1:200-1:400.
Immunohistochemistry 1:200-1:800. See general protocols online for guidelines.
Works on paraffin embedded tissue sections.
FACS: See online protocol.
Final working dilutions and protocols must be determined by end user.
Physical form
Protein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative.
Storage and Stability
Maintain for 1 year at 2–8°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Other Notes
Cytomegalovirus Control Slides, Catalogue Number 5027-5
Disclaimer
This product is intended for use by IVD manufacturers.
Contact customer service for assistance: oem.reagents@emdmillipore.com
Contact customer service for assistance: oem.reagents@emdmillipore.com
Minimum order quantity required to qualify. Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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