MAB1910
Anti-Collagen Type IV α 2 Chain Antibody, clone 23IIC3
ascites fluid, clone 23IIC3, Chemicon®
Synonym(s):
Anti-BSVD2, Anti-ICH, Anti-POREN2
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
ascites fluid
antibody product type
primary antibodies
clone
23IIC3, monoclonal
species reactivity
monkey, human, rat
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
isotype
IgG1
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... COL4A2(1284)
Specificity
Recognizes Human type IV collagen, alpha-2 chains.
Immunogen
Human amniotic type IV collagen
Application
Detect Collagen Type IV α 2 Chain using this Anti-Collagen Type IV α 2 Chain Antibody, clone 23IIC3 validated for use in ELISA, IH(P) & WB.
Immunohistochemistry: 1:500, acetone fixed, frozen tissue recommended. Weak reactivity on on formalin fixed, paraffin sections (1:100) after treatment with 1% trypsin (20 minutes, room temperature). Or alternatively, 4% PFA fixed, HEIR treated tissue has worked in monkey (Qin, 2003) at 1:200 dilution.
Western blot: 1:500, 170 kDa.
ELISA at 1:200
Optimal working dilutions must be determined by the end user.
Western blot: 1:500, 170 kDa.
ELISA at 1:200
Optimal working dilutions must be determined by the end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
ECM Proteins
ECM Proteins
Target description
170 kDa
Physical form
Liquid.
Unpurified
Storage and Stability
Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Analysis Note
Control
Positive Control: Kidney, muscle, tendon spleen tissue
Negative Control: Neurons/glia
Positive Control: Kidney, muscle, tendon spleen tissue
Negative Control: Neurons/glia
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Peter Trosan et al.
PloS one, 13(3), e0194820-e0194820 (2018-03-28)
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