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GF156

Sigma-Aldrich

B18R, Human Recombinant Carrier-Free

liquid, >98% (SDS-PAGE), <0.05 ng/μg cytokine (LAL test)

Synonym(s):

B18R protein, Recombinant B18R

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About This Item

UNSPSC Code:
12352202
eCl@ss:
32160405
NACRES:
NA.75

biological source

human

Quality Level

100

Assay

>98% (SDS-PAGE)

form

liquid

specific activity

≥1.3 x 10e6 units/mg protein

mol wt

Mw 38,389 Da

concentration

0.5 mg/mL

technique(s)

cell culture | stem cell: suitable

impurities

<0.05 ng/μg cytokine (LAL test)

input

sample type induced pluripotent stem cell(s)

solubility

water: soluble

suitability

suitable for molecular biology

shipped in

dry ice

General description

Product Source: Insect cells infected with baculovirus: amino acids His20-Glu 351 with a His-tag attached of Vaccinia Virus B18R.
Research area: Immuno and CKS B18R is a virally-encoded protein that acts as a decoy receptor for Type 1 Interferons. B18R enables increased cell viability during RNA transfection protocols designed to convert human somatic donor cells into iPSCs via direct delivery of synthetic mRNA′s. As a type I IFN receptor, B18R has a broad species specificity. It has high affinity for human IFN-alpha and also binds rabbit, bovine, rat, pig, and mouse IFN-alpha and IFN-beta.

Application

B18R, Human RecombinantCarrier-Free has been used as a decoy receptor to study the intrinsic antiviralactivity of recombinant soluble IFNα/β cell surface receptor 2 (sIFNAR2). Ithas also been used in the generation and maintenance of induced pluripotentstem cells (iPSCs) and human IPSCs.

Physical form

Sterile liquid;10 mM phosphate, 0.5 M NaCl, pH 7.5 buffer and is filtered with a 0.2µm filter before vialing.

Storage and Stability

For best recovery, quick-spin vial prior to opening. Thaw on ice. While on ice, aliquot B18R protein into sterile, nuclease-free, low protein-binding eppendorf tubes and store at -80°C. Limit repeated freeze and thaw cycle. Use in a sterile environment.
Further dilutions should be made into appropriate buffer or medium.

Analysis Note

The DTT-reduced protein migrates as a 48 kDa polypeptide on SDS-PAGE due to glycosylation. The non-reduced protein migrates as a 46 kDa polypeptide on SDS-PAGE due to internal cystines.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Soluble receptors of cytokines are able to modify cytokine activities and therefore the immune system, and some have intrinsic biological activities without mediation from their cytokines. The soluble interferon beta (IFN-ß) receptor is generated through alternative splicing of IFNAR2 and
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