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AP106P

Sigma-Aldrich

Rabbit Anti-Goat IgG Antibody, HRP conjugate

1 mg/mL (after reconstitution), Chemicon®

Synonym(s):

Goat IgG Detection, Goat IgG Detection Antibody, HRP Rabbit Anti-Goat IgG

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.46

biological source

rabbit

Quality Level

conjugate

peroxidase conjugate

antibody form

affinity purified immunoglobulin

antibody product type

secondary antibodies

clone

polyclonal

species reactivity

goat

manufacturer/tradename

Chemicon®

concentration

1 mg/mL (after reconstitution)

technique(s)

ELISA: suitable
immunohistochemistry: suitable
western blot: suitable

isotype

IgG

shipped in

wet ice

target post-translational modification

unmodified

Related Categories

General description

Affinity purified rabbit anti-goat IgG, Peroxidase conjugated.

Specificity

Specific for goat IgG, heavy and light chain.

Application

ELISA and Western Blots:
1:5,000-1:100,000 dilution can be used.

Immunohistochemistry:
1:500-1:5,000 dilution can be used.

Optimal working dilutions must be determined by end user.
Rabbit anti-Goat IgG Antibody, HRP conjugate is an antibody against Goat IgG for use in ELISA, IH & WB.
Research Category
Secondary & Control Antibodies
Research Sub Category
Whole Immunoglobulin Secondary Antibodies

Physical form

Immunoaffinity chromatography using antigen coupled to sepharose beads.
Purified Rabbit IgG conjugated to horseradish peroxidase in buffer containing 0.01M PBS,  pH 7.1, with 15mg/mL BSA. Lyophilized.

Storage and Stability

Lyophilized product is stable at 2-8°C for up to 12 months from date of shipment.
After reconstitution the product is stable for several weeks at 2-8°C as an undiluted liquid. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles.
WARNING: Use of sodium azide as a preservative will substantially inhibit the enzyme activity of HRP.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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The influence of trout cardiac troponin I and PKA phosphorylation on the Ca2+ affinity of the cardiac troponin complex.
Kirkpatrick KP, Robertson AS, Klaiman JM, Gillis TE.
The Journal of Experimental Biology null
Rebeca López-Úbeda et al.
PloS one, 10(6), e0130128-e0130128 (2015-06-23)
Gene Expression Microarray technology was used to compare oviduct transcriptome between inseminated and non-inseminated pigs during spontaneous oestrus. We used an in vivo model approaching the study from a physiological point of view in which no hormonal treatment (animals were
The influence of pKA treatment on the ca2+ activation of force generation by trout cardiac Muscle.
Gillis TE, Klaiman JM
The Journal of Experimental Biology null
M Shibata et al.
Journal of animal science, 87(8), 2700-2708 (2009-05-08)
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Leptin resistance following over-expression of protein tyrosine phosphatase 1B in liver.
N T Lam, S D Covey, J T Lewis, S Oosman, T Webber, E C Hsu, A T Cheung, T J Kieffer
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