Yeast VH1-related phosphatase (YVH1), also known as “dual specificity protein phosphatase 12” (DUSP12) is a ubiquitous phosphatase, with relatively high levels of expression in the ovary, testis, spleen, and peripheral blood leukocytes. YVH1 primarily removes phosphate groups from phosphorylated serine, threonine, and tyrosine residues in its target substrates. However, YVH1 also contains a zinc-binding domain which may enable it to interact with other proteins involved in cell growth, cell survival, and cell cycle progression. YVH1 may also be involved in the assembly of ribosomes, and in the biogenesis of the 60S ribosomal subunit. It can be targeted to different subcellular locations by phosphorylation of its Ser335 residue located in the zinc-binding domain.
Immunogen
GST-tagged recombinant protein corresponding to human YVH1.
Application
Anti-YVH1 Antibody is an antibody against YVH1 for use in Western Blotting.
Research Category Signaling
Research Sub Category Kinases & Phosphatases
Western Blot Analysis: A representative lot detected YVH1 in 10 µg of Jurkat cell lysate.
Quality
Evaluated by Western Blot in HeLa cells.
Western Blot Analysis: A 1:500 dilution of this antibody detected YVH1 in 10 µg of HeLa cell lysate.
Target description
~38 kDa observed. An uncharacterized band at ~36 kDa may be observed in some cell lysates.
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control HeLa cells
Disclaimer
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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