ABE208
Anti-β-Catenin Antibody
from rabbit, purified by affinity chromatography
Synonym(s):
catenin (cadherin-associated protein), beta 1, 88kDa, catenin (cadherin-associated protein), beta 1 (88kD)
Sign Into View Organizational & Contract Pricing
All Photos(3)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
mouse, human, rat, horse
species reactivity (predicted by homology)
ox (based on 100% sequence homology), equine (based on 100% sequence homology)
technique(s)
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
horse ... Ctnnb1(100055241)
human ... CTNNB1(1499)
mouse ... Ctnnb1(539003) , Ctnnb1(12387)
rat ... Ctnnb1(84353)
General description
β-catenin is the founding member of the β -catenin family. This protein is comprised of 12 ARM repeats and is considered to have dual function depending on its localization. Cytoplasmic β -catenin is a critical component of E-cadherin/catening adhesion complexes and is involved in regulating cadherin-dependent cell-cell interactions.When localized to the nucleus, β -catenin serves a regulatory role by binding with transcription factors from the TCF/LEF family, activating transcription. Expression of β -catenin has been reported in a variety of hair follicle cells such as cells found in the inner and outer portions of the root sheaths, and the basal and peripheral matrix cells. It has also been observed in colon tissue. Defects in expression have been linked to the development of several tumors and cancers such as; colorectal cancer (CRC), medulloblastoma (MDB), ovarian cancer (OC), and pilomatrixoma (PTR).
Specificity
Antibody recognizes β-Catenin at the N-terminus.
Immunogen
Epitope: Consensus GSK3 phosphorylation site of human β-Catenin
KLH-conjugated linear peptide corresponding to the consensus GSK3 phosphorylation site of human β-Catenin.
Application
Anti-β-Catenin Antibody is a Rabbit Polyclonal Antibody for detection of β-Catenin also known as catenin (cadherin-associated protein) beta 1 (88kD) & has been validated in WB, ICC & IP.
Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated β-Catenin from A431 lysate.
Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected β-Catenin in NIH/3T3 and A431 cells.
Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected β-Catenin in NIH/3T3 and A431 cells.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Transcription Factors
Quality
Evaluated by Western Blot in A431 cell lysate.
Western Blot Analysis: 0.05 µg/mL of this antibody detected β-Catenin on 10 µg of A431 cell lysate.
Western Blot Analysis: 0.05 µg/mL of this antibody detected β-Catenin on 10 µg of A431 cell lysate.
Target description
~86kDa observed.
Linkage
Replaces: 04-1002
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
A431 cell lysate
A431 cell lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Not finding the right product?
Try our Product Selector Tool.
recommended
Product No.
Description
Pricing
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Shuai Yuan et al.
Cell cycle (Georgetown, Tex.), 21(2), 187-201 (2021-12-18)
The present study aimed to investigate the role of neohesperidin (NH) in mice with steroid-induced femoral head necrosis (SONFH) and in bone marrow stromal cells (BMSCs). The SONFH model was established. The effects of NH on SONFH mice were detected
Daniel T Malleske et al.
Stem cells (Dayton, Ohio), 36(12), 1905-1916 (2018-09-02)
The wingless/integrase-1 (WNT)/β-catenin signaling pathway is active in several chronic lung diseases including idiopathic pulmonary fibrosis, asthma, and chronic obstructive pulmonary disease. Although this WNT/β-catenin pathway activity is associated with an increase in mucus cell frequency and a decrease in
Mi-Hyeon You et al.
Cells, 10(11) (2021-11-28)
The activation of metastatic reprogramming is vital for cancer metastasis, but little is known about its mechanism. This study investigated the potential role of death-associated protein kinase 1 (DAPK1) in thyroid cancer progression. We generated knockdown (KD) DAPK1 using siRNA
Sanna Vattulainen-Collanus et al.
Journal of cell science, 129(4), 693-705 (2016-01-09)
Tie2-promoter-mediated loss of peroxisome proliferator-activated receptor gamma (PPARγ, also known as PPARG) in mice leads to osteopetrosis and pulmonary arterial hypertension. Vascular disease is associated with loss of PPARγ in pulmonary microvascular endothelial cells (PMVEC); we evaluated the role of
Zejian Liu et al.
The Journal of biological chemistry, 291(15), 8059-8069 (2016-02-14)
The transport of monocarboxylate fuels such as lactate, pyruvate, and ketone bodies across brain endothelial cells is mediated by monocarboxylic acid transporter 1 (MCT1). Although the canonical Wnt/β-catenin pathway is required for rodent blood-brain barrier development and for the expression
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service