Skip to Content
Merck
All Photos(3)

Documents

ABE154

Sigma-Aldrich

Anti-Histone H3.1/H3.2 Antibody

from rabbit, purified by affinity chromatography

Synonym(s):

Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/I, Histone H3/j, Histone H3/k, Histone H3/l

Sign Into View Organizational & Contract Pricing
All Photos(3)

About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human, mouse

technique(s)

ChIP: suitable (ChIP-seq)
western blot: suitable

NCBI accession no.

NP_003520

UniProt accession no.

P68431

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... HIST1H3F(8968)

General description

Histone H3.1 and H3.2 are closely related histone variants that differ by only one amino acid. Mammalian cells express three major types of noncentromeric histone H3 variants, H3.1, H3.2, and H3.3. H3.1 is only expressed in mammals, while H3.2 is found in all eukaryotes except for yeast. Recent evidence shows that these nearly identical histone proteins differ in their expression profiles and post-translational modifications (PTMs). H3.2 is enriched in modifications associated with transcriptional silencing (di- and tri-methylation of K27), whereas H3.1 is enriched in both K9 dimethylation and K14 acetylation, suggesting that these variants may have separate biological functions.

Specificity

This antibody is expected to demonstrate broad species reactivity.

Immunogen

KLH-conjugated linear peptide corresponding to human Histone H3.1 and H3.2.

Application

Anti-Histone H3.1/H3.2 Antibody is a highly specific rabbit polyclonal antibody, that targets Histone H3 & has been tested in western blotting & ChIP-seq.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histones
Western Blotting Analysis: A representative lot detected Histone H3.2 in recombinant Histone H3.2 and mouse Embryonic Stem cell lysates.

Chromatin Immunprecipitation: A representative lot detected Histone H3.2 in mouse Embryonic Stem cell lysates (Elsasser, S. Graduate Thesis (http://hdl.handle.net/10209/440), Laboratory of Prof. David Allis, Rockefeller University).

Chromatin Immunoprecipitation Sequencing: A representative lot detected Histone H3.1/H3.2 in mouse Embryonic Stem cell lysates (Elsasser, S. Graduate Thesis (http://hdl.handle.net/10209/440), Laboratory of Prof. David Allis, Rockefeller University).

Quality

Evaluated by Western Blotting in HeLa acid extract.

Western Blotting Analysis: 0.5 µg/mL from a representative lot detected Histone H3.1 and H3.2 in 10 μg of HeLa acid extract.

Target description

~17 kDa observed. Uncharacterized bands may be observed at ~25 kDa and ~50 kDa

Physical form

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
HeLa acid extract

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Product Selector Tool.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Yuchen Zhang et al.
mBio, 11(5) (2020-10-29)
Epstein-Barr virus (EBV) infects 95% of adults worldwide and causes infectious mononucleosis. EBV is associated with endemic Burkitt lymphoma, Hodgkin lymphoma, posttransplant lymphomas, nasopharyngeal and gastric carcinomas. In these cancers and in most infected B-cells, EBV maintains a state of
Simon J Elsässer et al.
Nature, 522(7555), 240-244 (2015-05-06)
Transposable elements comprise roughly 40% of mammalian genomes. They have an active role in genetic variation, adaptation and evolution through the duplication or deletion of genes or their regulatory elements, and transposable elements themselves can act as alternative promoters for
Qiang Liu et al.
Frontiers in cell and developmental biology, 9, 626927-626927 (2021-03-06)
Little is known about the molecular relationships among follicle stimulating hormone (FSH), lipid droplet (LD) degradation, and autophagy. In this study, we aimed to investigate the pathway by which FSH regulates autophagy and the potential role of autophagy in progesterone
Irati Hervás-Corpión et al.
Cancers, 13(21) (2021-11-14)
Glioblastoma (GB) is the most aggressive form of glioma and is characterized by poor prognosis and high recurrence despite intensive clinical interventions. To retrieve the key factors underlying the high malignancy of GB with potential diagnosis utility, we combined the
Sara Martire et al.
Nature genetics, 51(6), 941-946 (2019-06-04)
The histone variant H3.3 is enriched at enhancers and active genes, as well as repeat regions such as telomeres and retroelements, in mouse embryonic stem cells (mESCs)1-3. Although recent studies demonstrate a role for H3.3 and its chaperones in establishing
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service