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For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf
324506
EDTA, 0.5 M, pH 8.0, Molecular Biology Grade, DEPC-Treated
Sterile-filtered solution of 0.5 M EDTA in H2O treated with diethyl pyrocarbonate (DEPC). Suitable for use in molecular biology applications.
Synonym(s):
EDTA, 0.5 M, pH 8.0, Molecular Biology Grade, DEPC-Treated, Ethylenediaminetetraacetic Acid Trisodium Salt
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About This Item
Recommended Products
grade
Molecular Biology
Quality Level
form
liquid
reaction suitability
reagent type: chelator
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
pH
8
shipped in
ambient
storage temp.
15-25°C
SMILES string
[Na+].[Na+].[Na+].N(CCN(CC(=O)[O-])CC(=O)O)(CC(=O)[O-])CC(=O)[O-].O.O.O
InChI
1S/C10H16N2O8.3Na.3H2O/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;;;;;;/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);;;;3*1H2/q;3*+1;;;/p-3
InChI key
FXNQQEVEDZAAJM-UHFFFAOYSA-K
General description
Application
- Comparing extraction method efficiency for high-throughput palaeoproteomic bone species identification.: This research compares various extraction methods for palaeoproteomic analysis, including those utilizing EDTA, to identify bone species efficiently. The study underscores the importance of EDTA in enhancing protein extraction for proteomic studies (Mylopotamitaki et al., 2023).
- Deep coverage and quantification of the bone proteome provides enhanced opportunities for new discoveries in skeletal biology and disease.: The use of EDTA in the preparation and extraction of bone samples for proteomic analysis is discussed, emphasizing its role in achieving deep proteomic coverage and facilitating new discoveries in skeletal biology (Rose et al., 2023).
Warning
Legal Information
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Dam. 1 - STOT RE 2 Inhalation
Target Organs
Respiratory Tract
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Certificates of Analysis (COA)
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How can I determine the shelf life / expiration / retest date of this product?
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How is shipping temperature determined? And how is it related to the product storage temperature?
1 answer-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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Hi, Is the DEPC innactivated in this solution ? I have some leftover and I am wondering if it would be suitable for use in a cell culture buffer context ? Thank you in advance for your support, Kind regards, Coline
1 answer-
Yes, DEPC was inactivated. Note that one of the break down products of DEPC is ethanol. DEPC-treated solutions are typically not used in cell culture applications and may be cytotoxic.
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Dear all, Is the EDTA 0.5 M solution prepared with before DEPC treated H2O, or, is it prepared together with DEPC and then autoclaved? Many thanks for your answer! Best, Julia
1 answer-
The solution is prepared with DEPC-treated H2O.
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