235474

Acridine Orange base

Dye content 75 %

• Synonym(s): 3,6-Bis(dimethylamino)acridine, Solvent Orange 15
• Empirical Formula (Hill Notation): C₁₇H₁₉N₃
• CAS Number: 494-38-2
• Molecular Weight: 265.35
• Colour Index Number: 46005
• MDL number: MFCD00005029
• UNSPSC Code: 12171500
• PubChem Substance ID: 24854106
• NACRES: NA.47

Properties

• form: powder
• composition: Dye content, 75%
• mp: 165 °C (dec.) (lit.)
• solubility: 1 M HCl: 1 mg/mL, clear
• λmax: 488 nm
• ε (extinction coefficient): ≥14000 at 227-233 nm; ≥20000 at 289-295 nm; ≥35000 at 486-492 nm; ≥40000 at 266-272 nm
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: CN(C)c1ccc2cc3ccc(cc3nc2c1)N(C)C
• InChI: 1S/C17H19N3/c1-19(2)14-7-5-12-9-13-6-8-15(20(3)4)11-17(13)18-16(12)10-14/h5-11H,1-4H3
• InChI key: DPKHZNPWBDQZCN-UHFFFAOYSA-N

Description

General description

Acridine Orange base (3,6-Bis(dimethylamino)acridine) is a cell-permeable fluorescent dye that binds to nucleic acids. It emits green fluorescence when bound to dsDNA and red fluorescence when bound to ssDNA or RNA. It is also known to be a positive solvatochromic dye.

Application

Acridine orange has also been used as a lysosomal dye. It is widely used to detect the presence of homeopathic potencies.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves