T9028

Monoclonal Anti-Tubulin, Tyrosine antibody produced in mouse

clone TUB-1A2, ascites fluid

• Synonym(s): Anti-CDCBM6, Anti-CSCSC1, Anti-M40, Anti-OK/SW-cl.56, Anti-TUBB1, Anti-TUBB5
• MDL number: MFCD00164753
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: unconjugated
• antibody form: ascites fluid
• antibody product type: primary antibodies
• clone: TUB-1A2, monoclonal
• contains: 15 mM sodium azide
• species reactivity: human, plant, animal
• technique(s): indirect immunofluorescence: 1:800 using cultured chicken fibroblasts; microarray: suitable; western blot: suitable
• isotype: IgG3
• UniProt accession no.: P07437
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... TUBA4A(7277) , TUBB(203068)

Description

General description

Monoclonal Anti-Tyrosine Tubulin (mouse IgG3 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Tubulin as a cylindrical filamentous structure and is present in almost all eukaryotic cells. Tubulin is a heterodimer which consists of α-tubulin and β-tubulin; both subunits have a molecular weight of 55 kDa and share considerable homology.

The intracellular cylindrical filamentous structure, microtubules is mainly made up of tubulin and is present in all eukaryotic cells. Monoclonal Anti-Tyrosine antibody can be used in immunocytochemical localization of tyrosinated α tubulin by indirect immunofluorescence labelling. Monoclonal Anti-Tyrosine antibody reacts specifically with tyrosine tubulin of bovine brain, African Green Monkey kidney cells (Vero), dog kidney (MDCK), marsupial kidney (Potoroo, PtK2), mouse pituitary tumour (AtT-20), yeast, and Xenopus.

Specificity

The antibody reacts against tubulin′s C-terminal tyrosine in immunoblotting assays and may be used for localization of this epitope in cultured cells or tissue sections.

Immunogen

peptide containing the carboxy-terminal amino acids of α-tubulin

Application

Monoclonal Anti-Tyrosine Tubulin has been used in:

• indirect immunofluorescent labelling
• immunoblotting technique
• immunocytochemical staining

Biochem/physiol Actions

Tubulin acts as a major building block of microtubules. Tubulin tyrosinylation is involved in the assembly status of tubulin. A specific tubulinyl tyrosine carboxypeptidase removes the terminal tyrosine to yield an α-tubulin terminating in a glutamic acid residue while another enzyme modifies the α-tubulin by addition of tyrosine to the carboxy terminus to offer a potential cycle of tyrosine addition and loss.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1