Skip to Content
Merck
All Photos(1)

Key Documents

View All Documentation

MAK115

Sigma-Aldrich

Hemoglobin Assay Kit

sufficient for 250 colorimetric tests

Synonym(s):

Hemoglobin Quantitation Kit

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
12161503
NACRES:
NA.84
Pricing and availability is not currently available.

usage

sufficient for 250 colorimetric tests

detection method

colorimetric

relevant disease(s)

hematological disorder

storage temp.

2-8°C

Gene Information

human ... HBA1(3039) , HBA2(3040) , HBB(3043)
mouse ... HBB(15129)
rat ... HBB(24440)

General description

Hemoglobin (Hb) is an iron-containing metalloprotein that serves as the primary means of oxygen transport in vertebrates. Hemoglobin is primarily found in red blood cells[1] where it makes up to 97% of the cell′s dry content. Hemoglobin can also be found in other tissues where it serves as an antioxidant. Alterations in blood hemoglobin levels occurs in many diseases such as anemia and polycytheima.[2][3]

Application

Hemoglobin Assay Kit has been used to measure the concentration of hemoglobin.[4][5]

Features and Benefits

Compatible with high-throughput handling systems. Can be adapted for use with cuvettes.

Suitability

Suitable for the detection of hemoglobinin a variety of samples such as blood, serum, plasma, and urine. This kit can be used to study the effects of various compounds on hemoglobin metabolism and levels.

Principle

This assay is based on the improved Triton™[NP1]/NaOH method in which hemoglobin is converted to a colorimetric product measured at 400 nm. This assay has a linear detection range between 0.9-200 mg/dL in the 96 well plate assay.

Legal Information

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Pictograms

CorrosionEnvironment
GHS05,GHS09

Signal Word

Warning

Hazard Statements

H290,H319,H410

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 2 - Eye Irrit. 2 - Met. Corr. 1

Storage Class Code

8B - Non-combustible corrosive hazardous materials

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
115CE11A22
115CE07A03
115CE02A22
115CE07A03
115CE11A22

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Malthe Hvas et al.
Comparative biochemistry and physiology. Part A, Molecular & integrative physiology, 255, 110913-110913 (2021-02-02)
In this study, Atlantic salmon post smolts (~250 g, ~29 cm) were fasted for four weeks at 12 °C in full strength seawater. During this period, the critical swimming speed (Ucrit) was measured after 1, 2 and 4 weeks of fasting, as well as
Viviana Lo Martire et al.
Frontiers in physiology, 9, 1818-1818 (2019-01-09)
Restless legs syndrome (RLS) is a neurological disorder that entails an urge to move with a circadian pattern during the evening/night. RLS may be accompanied by decreased sleep time and increased occurrence of periodic leg movements during sleep (PLMS), which
Heechun Kwak et al.
Research and practice in thrombosis and haemostasis, 4(8), 1301-1312 (2020-12-15)
Replacement therapy is the most common treatment for reduction of bleeding and control of episodic bleeding in individuals with hemophilia. Despite the proven effectiveness of factor replacement therapy, repeated intravenous administration is a heavy burden to individuals with hemophilia. To
Hemoglobin expression in nonerythroid cells: novel or ubiquitous?
Saha D, et al.
International Journal of Inflammation, 2014 (2014)
Benefits of fiber and antioxidant power of rice bran in Vitamin E deficient rats.
Carlos A G, et al.
Acta Bioquimica Clinica Latinoamericana, 51(1), 95-105 (2017)
View All Related Papers

Questions

1–4 of 4 Questions  

  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  3. Is there stability information available for the Reagent (MAK115A) and Calibrator (MAK115B) included in the hemoglobin assay kit, especially after they are opened?

    1 answer

    1. The recommended storage for both components is at 2-8 degrees Celsius when not in use. Cycling the components between refrigerated and room temperature may decrease shelf life, but there is no predicted rate of degradation. For prolonged shelf life with frequent use, aliquoting the reagents into smaller tubes for individual use is advised.

      Helpful?

  4. What specific items are included in this kit? Is there a standardized hemoglobin assay?

    1 answer

    1. There is a hemoglobin calibrator included with the kit, and the concentration of the sample is determined relative to the known concentration of the calibrator.

      Helpful?

Reviews

No rating value

Active Filters

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service