H7665

Anti-Histone H1.4 antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonym(s): Anti-H1 Histone family, member 4, Anti-H1.4, Anti-H1F4, Anti-HIST1H1E, Anti-Histone H1b, Anti-Histone I family, 4, Anti-Histone cluster 1 H1e
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~35 kDa
• species reactivity: human
• concentration: ~1.0 mg/mL
• technique(s): western blot: 1-2 μg/mL using acid-extracted fraction of HL60 cells
• UniProt accession no.: P10412
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... HIST1H1E(3008)

Description

General description

In mammalian cells, four histone H1 variants (H1.2 to H1.5) are found in all somatic cells, and a fifth (H1.1) is present in thymus, testis and spleen, lymphocytic and neuronal cells. K26/H1.4 is present within the flexible N-terminal domain of H1.4 just before the globular domain. Histone modifications are thought to play an important role in cancer and disease.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Biochem/physiol Actions

Linker histone H1 binds to DNA between nucleosomal core particles and is involved in establishing and maintaining higher order chromatin structures. It is covalently modified. Histone H1 phosphorylation occurs at multiple sites including at Ser²⁷ residue. Histone H1.4 is di-methylated or acetylated at Lys²⁶. enhancer of Zeste 2 (Ezh2).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves