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F9182

Sigma-Aldrich

FSL-biotin

Synonym(s):

FSL-CONJ(1Biotin)-SC2-L1

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About This Item

Empirical Formula (Hill Notation):
C91H149N17NaO32PS
Molecular Weight:
2079.28
UNSPSC Code:
12352200
NACRES:
NA.32
Pricing and availability is not currently available.

storage temp.

−20°C

Quality Level

200

Application

FSL Constructs Video Protocol
All FSL constructs disperse in biocompatible media and spontaneously and stably incorporate into cell membranes. Cells modified with FSL constructs are known as kodecytes and usually maintain their normal vitality and functionality. FSL-biotin has been specifically designed to create biotinylated live cells (kodecytes) but can also be used to modify other hydrophobic surfaces including fixed cells, virions, and solid phase surfaces. Biotinylated kodecytes can be reacted with a variety of biotin binding protein constructs including fluorescent labeled avidin and avidinylated beads/solid surfaces.
All FSL constructs disperse in biocompatible media and spontaneously and stably incorporate into cell membranes. FSL-biotin has been specifically designed to create biotinylated live cells (kodecytes) but can also be used to modify other hydrophobic surfaces including fixed cells, virions, and solid phase surfaces.

Other Notes

FSL-biotin is a KODE™ technology construct designed to label hydrophobic surfaces including living cells with biotin. All FSL constructs consist of three essential designable features: a functional component (F), a spacer (S), and a diacyl lipid (L). FSL-biotin is comprised of a monomer of biotin (vitamin B7) representing F, conjugated to a maleimide-bearing carboxymethylglycine based linker (SC2) in turn conjugated to an activated adipate derivative of dioleoylphosphatidylethanolamine (L1).

Legal Information

KODE Constructs are supplied by KODE Biotech Materials Limited under license from KODE Biotech Limited.
KODE is a trademark of KODE Biotech Ltd.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000410111
0000410111
SLCN3800
SLCL0629
SLCJ8507

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Questions

1–7 of 7 Questions  

  1. Will the Product F9182, FSL-biotin, label dead cells?

    1 answer

    1. Product F9182, FSL-biotin, is designed to insert into the membranes of live cells. But it can also be used to label fixed cells, enveloped virions, and solid phase surfaces.

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  2. How does the storage temperature relate to shipping conditions?

    1 answer

    1. The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment.

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  3. May I use serum with the product F9182, FSL-biotin?

    1 answer

    1. We do not recommend diluting the Product F9182, FSL-biotin, in lipid-containing buffers. The FSL will associate with the lipids and reduce the efficiency of insertion into cell membranes.

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  4. How many cells can I label with the product F9182, FSL-biotin?

    1 answer

    1. The exact number of cells may vary based on cell type and cell condition.  The recommended concentration to use for labeling is 10-100ug/ml (~4.8-48mM), which is mixed in a 1:1 volume with cells.

      Helpful?

  5. What concentration of F9182, FSL-biotin do I use to label cells?

    1 answer

    1. For cell labeling we recommend using a concentration of 10-100ug/ml of F9182, FSL-biotin diluted in PBS. Label cells with FSL-biotin at a 1:1 volume to volume ratio. The exact concentration may need to be optimized for the cells and the application.

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  6. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  7. What should I use to reconstitute Product F9182, FSL-biotin?

    1 answer

    1. We recommend reconstituting 1 mg of Product F9182, FSL-biotin with 0.5 mL of saline or PBS for a concentration of 2 mg/mL.

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