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F8648

Sigma-Aldrich

Monoclonal Anti-Interferon-γ−Carboxyfluorescein antibody produced in mouse

clone 25723.11, purified immunoglobulin, buffered aqueous solution

Synonym(s):

Monoclonal Anti-Interferon-γ

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About This Item

UNSPSC Code:
51111800
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

carboxyfluorescein conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

25723.11, monoclonal

form

buffered aqueous solution

species reactivity

human

technique(s)

flow cytometry: suitable

isotype

IgG2b

UniProt accession no.

shipped in

wet ice

storage temp.

2-8°C

Gene Information

human ... IFNG(3458)

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General description

Interferons (IFNs) are cytokines that are secreted in response to viral or bacterial infections and tumorigenesis. There are two types of IFNs, type I (IFN-α and IFN-β) and type II (IFN-γ). IFN-γ binds to specific receptor complex consisting of IFNγR1 and IFNγR2. There are many pathways that are mediated by IFN-γ binding such as JAK/STAT1, AP-1, NF-κB, STAT3 and STAT5. IFN-γ is pleotropic and performs various functions related to immune response, inflammation, differentiation and activation of T cells, cell cycle and apoptosis. The most studied function of IFN-γ is priming the antigen presenting cells by up regulating major histocompatibility (MHC) Class I molecules. IFN-γ has clinical applications in autoimmune diseases (rheumatoid arthritis), multiple sclerosis, cancer, HIV and fungal infections. Anti-Interferon-γ recognizes intracellular human IFN-γ. The product is prepared by conjugation of carboxyfluorescein to Protein G purified IFN-γ monoclonal antibody.

Specificity

The conjugates will identify and quantitate cells containing intracellular forms of human IFN-γ by flow cytometry.

Immunogen

recombinant human IFN-γ

Application

Anti-interferon-γ antibody may be used for detection of intracellular IGN-γ in peripheral blood lymphocytes by adding 10 μL of the antibody to 1-5 X 105 cells.

Physical form

Solution in phosphate buffered saline containing 0.1% sodium azide.

Preparation Note

Purified antibody is conjugated to carboxyfluorescein.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Adithya Cattamanchi et al.
Journal of acquired immune deficiency syndromes (1999), 56(3), 230-238 (2011-01-18)
To determine whether interferon-gamma release assays (IGRAs) improve the identification of HIV-infected individuals who could benefit from latent tuberculosis infection therapy. Systematic review and meta-analysis. We searched multiple databases through May 2010 for studies evaluating the performance of the newest
Leonidas C Platanias
Nature reviews. Immunology, 5(5), 375-386 (2005-05-03)
Interferons are cytokines that have antiviral, antiproliferative and immunomodulatory effects. Because of these important properties, in the past two decades, major research efforts have been undertaken to understand the signalling mechanisms through which these cytokines induce their effects. Since the
Kate Schroder et al.
Journal of leukocyte biology, 75(2), 163-189 (2003-10-04)
Interferon-gamma (IFN-gamma) coordinates a diverse array of cellular programs through transcriptional regulation of immunologically relevant genes. This article reviews the current understanding of IFN-gamma ligand, receptor, signal transduction, and cellular effects with a focus on macrophage responses and to a
S E Ealick et al.
Science (New York, N.Y.), 252(5006), 698-702 (1991-05-03)
The x-ray crystal structure of recombinant human interferon-gamma has been determined with the use of multiple-isomorphous-replacement techniques. Interferon-gamma, which is dimeric in solution, crystallizes with two dimers related by a noncrystallographic twofold axis in the asymmetric unit. The protein is
M Raza Zaidi et al.
Clinical cancer research : an official journal of the American Association for Cancer Research, 17(19), 6118-6124 (2011-06-28)
Interferon-γ is a cytokine whose biological activity is conventionally associated with cytostatic/cytotoxic and antitumor mechanisms during cell-mediated adaptive immune response. It has been used clinically to treat a variety of malignancies, albeit with mixed results and side effects that can

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