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51410

Sigma-Aldrich

Nε-(N-(+)-Biotinyl-6-aminohexanoyl)-Nα,Nα-bis(carboxymethyl)-L-lysine tripotassium salt

≥98.0% (TLC)

Synonym(s):

Biotin-X-NTA

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About This Item

Empirical Formula (Hill Notation):
C26H40K3N5O9S
CAS Number:
856661-92-2
Molecular Weight:
715.98
UNSPSC Code:
12352209
NACRES:
NA.32

Quality Level

200

Assay

≥98.0% (TLC)

form

solid

SMILES string

[K+].[K+].[K+].[O-]C(=O)CN(CC([O-])=O)[C@H](CCCCNC(=O)CCCCCNC(=O)CCCC[C@@H]1SC[C@@H]2NC(=O)N[C@H]12)C([O-])=O

Application

Nε-(N-(+)-Biotinyl-6-aminohexanoyl)-Nα,Nα-bis(carboxymethyl)-L-lysine (Biotin-X-NTA) is used as a hetero-bifunctional linker between histone(His)-tagged proteins and (strept)avidin conjugates.
Hetero-bifunctional linker for His-tagged proteins on one side and (strept)avidin conjugates

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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Igor L Medintz et al.
Langmuir : the ACS journal of surfaces and colloids, 21(12), 5501-5510 (2005-06-01)
This report describes two related methods for decorating cowpea mosaic virus (CPMV) with luminescent semiconductor nanocrystals (quantum dots, QDs). Variants of CPMV are immobilized on a substrate functionalized with NeutrAvidin using modifications of biotin-avidin binding chemistry in combination with metal
D J O'Shannessy et al.
Analytical biochemistry, 229(1), 119-124 (1995-07-20)
The use of short peptide affinity tag sequences has become commonplace for the expression and purification of recombinant proteins. Many of these tags are antibody epitopes and detection of tagged proteins via Western blots is straightforward. However, the most common
S A McMahan et al.
Analytical biochemistry, 236(1), 101-106 (1996-04-05)
Using a one-step reaction, a bifunctional compound was synthesized for detecting histidine-tagged proteins immobilized on nitrocellulose. This compound has a biotin as one functional group and a nitrilotriacetic acid as the other. The nitrilotriacetic acid is used to chelate a
Xiue Jiang et al.
Physical chemistry chemical physics : PCCP, 10(42), 6381-6387 (2008-10-31)
Surface enhanced infrared absorption spectroscopy (SEIRAS) has been employed to monitor the orientated assembly of a strep-tagged membrane protein on the gold surface via a streptavidin/biotin interlayer. The high surface sensitivity of SEIRAS allows for tracking the individual assembling steps
Eunah Kang et al.
Langmuir : the ACS journal of surfaces and colloids, 23(11), 6281-6288 (2007-04-21)
Silica surfaces modified with nitrilotriacetic acid (NTA)-polyethylene glycol (PEG) derivatives were used to immobilize hexahistidine-tagged green fluorescent protein (His6-GFP), biotin/streptavidin-AlexaFluor555 (His6-biotin/SA-AF), and gramicidin A-containing vesicles (His6-gA). Three types of surface-reactive PEG derivatives-NTA-PEG3400-Si(OMe)3, NTA-PEG3400-vinylsulfone, and mPEG5000-Si(OMe)3 (control)-were grafted onto silica and
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