09-271
Anti-Rac1b Antibody
Upstate®, from rabbit
Synonym(s):
Ras-related C3 botulinum toxin substrate 1, Cell migration-inducing gene 5 protein, Ras-like protein TC25, p21-Rac1
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All Photos(8)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
affinity purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
mouse, human
manufacturer/tradename
Upstate®
technique(s)
immunofluorescence: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... RAC1(5879)
General description
Ras-related C3 botulinum toxin substrate 1 (UniProt: P63000; also known as Cell migration-inducing gene 5 protein, Ras-like protein TC25, p21-Rac1) is encoded by the RAC1 (also known as TC25, MIG5) gene (Gene ID: 5879) in human. Rac1 is a plasma membrane-associated small GTPase that cycles between active GTP-bound and inactive GDP-bound states. In its active state, it binds to a variety of effector proteins to regulate cellular responses. Two isoforms of Rac1 have been described that are produced by alternative splicing. Rac1b differs from Rac1a in that it has an additional 19 amino acids and is found in various tumors, such as breasts and colorectal. Rac1b has an accelerated GEF-independent GDP/GTP exchange and an impaired GTP hydrolysis, which is restored partially by GTPase-activating proteins. It can bind to the GTPase-binding domain of PAK, but not full-length PAK in a GTP-dependent manner, suggesting that the insertion does not completely abolish effector interaction. Isoform B is predominantly identified in skin and epithelial tissues from the intestinal tract. Its expression is elevated in colorectal tumors at various stages of neoplastic progression, as compared to their respective adjacent tissues.
Specificity
This rabbit polyclonal antibody specifically detects Rac1b in human and murine cells.
Immunogen
KLH-conjugated linear peptide corresponding to 15 amino acids from the N-terminal half of human Rac1b.
Application
Anti-Rac1b Antibody, Cat. No. 09-271, is a rabbit polyclonal antibody that detects Ras-related C3 botulinum toxin substrate 1 (Rac1b) and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Rac1b in HeLa, A431, HUVEC, and NIH/3T3 cells.
Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative detected Rac1b in human colon cancer and human prostate cancer tissue sections.
Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative detected Rac1b in human colon cancer and human prostate cancer tissue sections.
Quality
Evaluated by Western Blotting in MCF7 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Rac1b in MCF7 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Rac1b in MCF7 cell lysate.
Target description
~17 kDa observed; 23.47 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physical form
Purified rabbit polyclonal antibody in PBS with 0.05% sodium azide and 30% glycerol.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Rac1b regulates NT3-stimulated Mek-Erk signaling, directing marrow-isolated adult multilineage inducible (MIAMI) cells toward an early neuronal phenotype.
Kevin M Curtis,Lourdes A Gomez,Paul C Schiller
Molecular and Cellular Neurosciences null
Christine Mehner et al.
Molecular cancer research : MCR, 12(10), 1430-1439 (2014-05-23)
Pancreatic ductal adenocarcinoma (PDA) arises at the convergence of genetic alterations in KRAS with a fostering microenvironment shaped by immune cell influx and fibrotic changes; identification of the earliest tumorigenic molecular mediators evokes the proverbial chicken and egg problem. Matrix
Hendrik Ungefroren et al.
Cancers, 13(6) (2021-04-04)
Autocrine transforming growth factor β (aTGFβ) has been implicated in the regulation of cell invasion and growth of several malignant cancers such as pancreatic ductal adenocarcinoma (PDAC) or triple-negative breast cancer (TNBC). Recently, we observed that endogenous TGFB1 can inhibit
Christine Mehner et al.
Genes & cancer, 6(11-12), 480-489 (2016-01-26)
Breast, lung, and pancreatic cancers collectively represent one third of all diagnosed tumors and are responsible for almost 40% of overall cancer mortality. Despite improvements in current treatments, efforts to develop more specific therapeutic options are warranted. Here we identify
Paula M Schmidtlein et al.
Cancers, 13(18) (2021-09-29)
Pancreatic ductal adenocarcinoma (PDAC) is one of the most aggressive and therapy-resistant cancer types which is largely due to tumor heterogeneity, cancer cell de-differentiation, and early metastatic spread. The major molecular subtypes of PDAC are designated classical/epithelial (E) and quasi-mesenchymal
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