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04-1565

Sigma-Aldrich

Anti-NSD1 Antibody, clone 1NW-1A10

ascites fluid, clone 1NW-1A10, from mouse

Synonym(s):

Androgen receptor-associated coregulator 267, Lysine N-methyltransferase 3B, NR-binding SET domain-containing protein, Nuclear receptor-binding SET domain-containing protein 1, Sotos syndrome, nuclear receptor binding SET domain protein 1

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

ascites fluid

antibody product type

primary antibodies

clone

1NW-1A10, monoclonal

species reactivity

rat

species reactivity (predicted by homology)

mouse (based on 100% sequence homology)

technique(s)

immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable

isotype

IgG1κ

NCBI accession no.

NP_032765.3

UniProt accession no.

O88491

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

mouse ... Nsd1(18193)
rat ... Nsd1(306764)

General description

NSD1 (also known as KMT3B) is a histone methyltransferase shown to methylate lysine 36 of histone H3 and lysine 20 of histone H4. NSD1 is a described as an intermediary transcription factor and depending on its cellular context, it is able to either negatively or positively influence transcription. Expression of this gene has been described in skeletal muscles, kidneys, adult and fetal brain, thymus and spleen. Heterozygous mutations and deletions of the NSD1 gene are associated with Sotos Syndrome, an autosomal dominant condition characterized by macrocephaly, tall stature, facial elongation, accelerated bone aging, and mental retardation.

Specificity

This antibody recognizes NSD1.

Immunogen

Epitope: Unknown
Recombinant protein corresponding to mouse NSD1.

Application

Anti-NSD1 Antibody, clone 1NW-1A10 is a mouse monoclonal antibody for detection of NSD1, also known as &rogen receptor-associated coregulator 267, Lysine N-methyltransferase 3B, has been validated in WB.
Research Category
Epigenetics & Nuclear Function

Epigenetics & Nuclear Function
Research Sub Category
Histone Modifying Proteins

Chromatin Biology
Used for immunohistochemistry on paraffin sections in Visser et al., (2012).
Used for fluorescent immunocytochemistry in Huang et al.,(1998).

Quality

Evaluated by Western Blot in PC12 cell lysate.

Western Blot Analysis: 1:1,000 dilution of this antibody detected NSD1 on 10 µg of PC12 cell lysate.

Target description

~ 300 kDa

Physical form

Unpurified
Unpurified mouse monoclonal IgG1κ in preservative-free ascites.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
PC12 cell lysate

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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N Huang et al.
The EMBO journal, 17(12), 3398-3412 (1998-06-17)
NSD1, a novel 2588 amino acid mouse nuclear protein that interacts directly with the ligand-binding domain (LBD) of several nuclear receptors (NRs), has been identified and characterized. NSD1 contains a SET domain and multiple PHD fingers. In addition to these
Sotos Syndrome Is Associated with Deregulation of the MAPK/ERK-Signaling Pathway.
Visser, Remco, et al.
Testing, 7, e49229-e49229 (2012)
Nur Zafirah Zaidan et al.
Nucleic acids research, 48(22), 12660-12674 (2020-11-26)
The heterochromatin protein 1 (HP1) family members are canonical effectors and propagators of gene repression mediated by histone H3 lysine 9 (H3K9) methylation. HP1γ exhibits an increased interaction with active transcription elongation-associated factors in embryonic stem cells (ESCs) compared to
H Nakshatri et al.
Cell death & disease, 6, e1608-e1608 (2015-01-23)
The transcription factor nuclear factor-kappaB (NF-κB) is constitutively active in several cancers and is a target of therapeutic development. We recently developed dimethylaminoparthenolide (DMAPT), a clinical grade water-soluble analog of parthenolide, as a potent inhibitor of NF-κB and demonstrated in

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