700147P

PhotoClick Cholesterol

Hex-5′-ynyl 3β-hydroxy-6-diazirinyl-5α-cholan-24-oate, powder

• Synonym(s): hex-5-yn-1-yl 4-((3S,5S)-3-hydroxy-10, 13-dimethyl-1,2,3,4,5,7,8,9,10,11,12,13,14,15,16,17-hexadecahydrospiro[cyclopenta[a]phenanthrene-6,3′-diazirin]-17-yl)pentanoate
• Empirical Formula (Hill Notation): C₃₀H₄₆N₂O₃
• CAS Number: 1485490-47-8
• Molecular Weight: 482.70
• UNSPSC Code: 12352211
• NACRES: NA.25

Properties

• Assay: >99% (TLC)
• form: powder
• packaging: pkg of 1 × 100 μg (with stopper and crimp cap (700147P-100ug)); pkg of 1 × 250 μg (with stopper and crimp cap (700147P-250ug))
• manufacturer/tradename: Avanti Research^™ - A Croda Brand 700147P
• lipid type: click reagents
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

PhotoClick Cholesterol is a clickable, photoreactive sterol probe for use in combination with quantitative mass spectrometry to globally map cholesterol-protein interactions directly in living cells. In addition to the UV-activatable cross-linking diazirine group that reacts with proteins, the sterol contains an alkyne group for incorporation of reporter and affinity tags through click-chemistry.

PhotoClick Cholesterol is a synthetic analog of cholesterol and is photoreactive. It comprises the alkyne group.

Application

PhotoClick Cholesterol has been used:

• in lysosome-associated membrane protein 1 (LAMP1) crosslinking studies
• as an analog of cholesterol in binding and competition assay of sterol binding proteins from yeast
• as a conjugate with the ectodomain of lysosomal integral membrane protein-2 (LIMP-2)

Biochem/physiol Actions

PhotoClick Cholesterol serves as a probe for tracking cholesterol-binding proteins. The copper-catalyzed azide-alkyne cycloaddition is the basis for its probe functionality. The alkyne group is essential for the click chemistry property. Its binding to partners is mediated by photocrosslinking.

Packaging

2 mL Amber Serum Vial with Stopper and Crimp Cap (700147P-100ug)

2 mL Amber Serum Vial with Stopper and Crimp Cap (700147P-250ug)

Legal Information

Avanti Research is a trademark of Avanti Polar Lipids, LLC

Safety Information

• Storage Class Code: 11 - Combustible Solids