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Sigma-Aldrich

ISOGRO®-15N Powder -Growth Medium

98 atom % 15N

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About This Item

MDL number:
MFCD04113754
UNSPSC Code:
12352200
NACRES:
NA.12
Pricing and availability is not currently available.

isotopic purity

98 atom % 15N

technique(s)

bio NMR: suitable
protein expression: suitable

storage temp.

−20°C

Related Categories

Application

ISOGRO®-15N Powder -Growth Medium has been used:
  • for the production of 15N histone H2A for nuclear magnetic resonance (NMR) spectroscopy studies.[1]
  • for the production of 15N-enriched protein kinase C[2] and H3 histone[3] for NMR studies.

Packaging

This product may be available from bulk stock and can be packaged on demand. For information on pricing, availability and packaging, please contact Stable Isotopes Customer Service.

Legal Information

ISOGRO is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
MBBD8596
MBBD3290
MBBC6497
MBBC0340V
MBBB1580V

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Expression and purification of the N-terminal regulatory domain of protein kinase C for biophysical studies
Cole TR and Igumenova TI
Protein Expression and Purification, 110(4), 14-21 (2015)
Mechanisms of ubiquitin-nucleosome recognition and regulation of 53BP1 chromatin recruitment by RNF168/169 and RAD18
Hu Q, et al.
Molecular Cell, 66(4), 473-487 (2017)
Christian Koehler et al.
The Journal of biological chemistry, 286(17), 14842-14851 (2011-03-04)
NarE is a 16 kDa protein identified from Neisseria meningitidis, one of the bacterial pathogens responsible for meningitis. NarE belongs to the family of ADP-ribosyltransferases (ADPRT) and catalyzes the transfer of ADP-ribose moieties to arginine residues in target protein acceptors.
Carissa L Perez et al.
Cell metabolism, 8(3), 266-274 (2008-09-03)
Although studies in C. elegans have identified numerous genes involved in fat storage, the next step is to determine how these factors actually affect in vivo lipid metabolism. We have developed a (13)C isotope assay to quantify the contribution of
Emma A Morrison et al.
The Journal of biological chemistry, 289(10), 6825-6836 (2014-01-23)
EmrE, a small multidrug resistance transporter, serves as an ideal model to study coupling between multidrug recognition and protein function. EmrE has a single small binding pocket that must accommodate the full range of diverse substrates recognized by this transporter.
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Articles

Utilizing ISOGRO® Supplementation of M9 Minimal Media to Enhance Recombinant Protein Expression

Utilizing ISOGRO® Supplementation of M9 Minimal Media to Enhance Recombinant Protein Expression.

Questions

1–2 of 2 Questions  

  1. What is the formulation of ISOGRO®-15N Powder?

    1 answer

    1. The nitrogen source is from green algae, not amino acids. The components are glucose, salts, water, nitrogen and carbon. The exact formulation is proprietary.

      Helpful?

  2. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

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