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TOYOPEARL® HW-55F Bulk Media

phase hydroxylated methacrylate, bottle of 500 mL, 45 μm particle size

Synonym(s):

TOYOPEARL® HW-55 Size Exclusion Media

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About This Item

UNSPSC Code:
41115700

description

HW-55F

form

beads

packaging

bottle of 500 mL

parameter

3 bar max. pressure

technique(s)

HPLC: suitable
LPLC: suitable

matrix

hydroxylated methacrylic polymer

matrix active group

hydroxylated methacrylate phase

particle size

45 μm

pore size

1000-200,000 Da MW range

operating pH

2-13

separation technique

size exclusion (SEC)

General description

Toyopearl HW-55 resin is a hydroxylated methacrylic polymer bead for size exclusion chromatography of proteins between 1,000-700,000 Da. It also is the base bead for all Toyopearl "-550" designated products.

Application

TOYOPEARL® media fractionate mixtures of proteins and other large molecular weight compounds over a wide size range. Applications include separating RNA from protein, resolving oligosaccharides by degree of polymerization, and isolating agglutinin while maintaining a high hemagglutination titer.

Specifications

Clean in place with 0.5 M NaOH or 0.1 M HCl.

Physical form

Shipped in 20% (v/v) ethanol.

Recommended products

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

Toyopearl is a registered trademark of Tosoh Corporation

Pictograms

Flame
GHS02

Signal Word

Danger

Hazard Statements

H225

Hazard Classifications

Flam. Liq. 2

Storage Class Code

3 - Flammable liquids

WGK

WGK 3

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Olga N Smol'kina et al.
Carbohydrate research, 347(1), 161-163 (2011-11-22)
A method is developed for the preparation of D-rhamnose from an O-polysaccharide (OPS) isolated by mild acid hydrolysis of Azospirillum brasilense SR75 cell mass. After the OPS hydrolysis, D-rhamnose was recovered by gel-permeation chromatography on Toyopearl TSK HW-40 and was
Shu-fen Xu et al.
Fitoterapia, 83(1), 153-160 (2011-11-01)
Persimmon proanthocyanidin was fractionated on Toyopearl TSK-HW-50-F to yield a fraction with strong inhibition on the catalytic activity and edema-inducing activity and lethality of Chinese cobra PLA(2). Thiolysis suggested that the terminal units included C, EGCG and myricetin, and epicatechin
M V Pokrovskaya et al.
Protein expression and purification, 82(1), 150-154 (2012-01-10)
We have cloned ansB (YPTB1411) gene from Yersinia pseudotuberculosis Q66CJ2 and constructed stable inducible expression system that overproduce L-asparaginase from Y. pseudotuberculosis (YpA) in Escherichiacoli BL21 (DE3) cells. For purification of YpA we used Q-Sepharose and DEAE-Toyopearl column chromatography. We

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