ZRB1519

Anti-RXR-alpha Antibody, clone 2I8 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): Nuclear receptor subfamily 2 group B member 1, Retinoic acid receptor RXR-alpha, Retinoid X receptor alpha
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit (recombinant)
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 2I8, monoclonal; recombinant monoclonal
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 50.81 kDa; observed mol wt ~49 kDa
• species reactivity: human, rat
• packaging: antibody small pack of 25 μL
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• technique(s): affinity binding assay: suitable; immunocytochemistry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• isotype: IgG
• UniProt accession no.: P19793
• greener alternative category: Aligned,
• shipped in: ambient
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... RXRA(6256)

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

Specificity

Clone 2I8 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Retinoid X receptor alpha. It targets an epitope within 16 amino acids from the N-terminal region.

Immunogen

KLH-conjugated linear peptide corresponding to 16 amino acids from the N-terminal region of human retinoid X receptor alpha.

Application

Anti-RXR-alpha, clone 2I8 ZooMAb, Cat. No. ZRB1519, is a recombinant Rabbit monoclonal antibody that targets RXR-alpha and is tested for use in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected RXR-alpha in MCF-7 cell lysate.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected RXR-alpha in HUVEC and HeLa cells.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected RXR-alpha in human skin and human esophagus tissue sections.

Affinity Binding Assay: A representative lot of this antibody bound RXR alpha peptide with a KD of 1.3 x 10-6 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Target description

Retinoic acid receptor RXR-alpha (UniProt: P19793; also known as Nuclear receptor subfamily 2 group B member 1, Retinoid X receptor alpha) is encoded by the RXRA (also known as NR2B1) gene (Gene ID: 6256) in human. RXR-alpha is a homodimeric protein that can also for heterodimer with RAR-alpha via its C-terminal. RXR-alpha serves as a receptor for retinoic acid and binds to target response elements in response to their ligands, all-trans or 9-cis retinoic acid, to regulate the expression of genes involved in various biological processes. 9-cis retinoic acid is considered as a high affinity ligand of RXR-alpha. The RAR/RXR heterodimers are reported to bind to the retinoic acid response elements (RARE) composed of tandem 5′-AGGTCA-3′ sites known as DR1-DR5 to regulate transcription. In the absence of ligand, the RXR-RAR heterodimers associate with a multiprotein complex containing transcription co-repressors that induce histone deacetylation, chromatin condensation and transcriptional suppression. On ligand binding, the corepressors dissociate from the receptors and coactivators are recruited leading to transcriptional activation. RXR-alpha is highly expressed in liver and its expression has also been reported in lung fibroblasts, monocytes, kidney, and brain. RXR-alpha is composed of three domains: a modulating N-terminal domain (AF1 domain), a DNA-binding domain, and a C-terminal ligand-binding domain (AF2 domain). Its DNA binding region is localized to amino acids 135-200. It has two NR-C4 type zinc-finger domains (aa 135-155 and 171-195). Its nuclear localization signal is localized to amino acids 160-165 and the region comprising of amino acids 348-367 is essential for its nuclear export. Its nuclear localization is enhanced by vitamin D3. Its acetylation by EP300 is shown to enhance its DNA-binding and transcriptional activity. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Chen, L., et al. (2018). Cell. Mol. Biol. Lett. 23; Article 36; Rastinejad, F., et al. (2000). EMBO J.19(5); 1045-1054).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

0.3 mg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable