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X4875

Sigma-Aldrich

Xanthine Oxidase from bovine milk

Grade IV, ammonium sulfate suspension, ≥0.1 units/mg protein

Synonym(s):

XOD, Xanthine:oxygen oxidoreductase

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10 UNITS
€72.60
40 UNITS
€224.00
120 UNITS
€582.00

€72.60

Estimated to ship on03 April 2025

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10 UNITS
€72.60
40 UNITS
€224.00
120 UNITS
€582.00

About This Item

CAS Number:
9002-17-9
Enzyme Commission number:
1.17.3.2.
EC Number:
232-657-6
MDL number:
MFCD00082145
UNSPSC Code:
12352204
NACRES:
NA.54

€72.60

Estimated to ship on03 April 2025

Request a Bulk Order

biological source

bovine milk

Quality Level

200

type

Grade IV

form

ammonium sulfate suspension

specific activity

≥0.1 units/mg protein

storage temp.

2-8°C

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General description

Formerly E.C. 1.1.3.22
Research area: Cell CycleXanthineoxidase (XO) is a homodimer. It is made up of two sub-units each made up of onemolybdenum and two iron-sulphur (2Fe-2S) centers.[1]

Application

Xanthine Oxidase from bovine milk has been used in superoxide dismutaseactivity assay.[2][3]Xanthine oxidase may be suitable for producing amperometric biosensors and designing-flavonoid-type inhibitors against XO for treating inflammatory diseases, gout, and hyperuricemia. It may also be used in the synthesis of superoxide because of aldehyde oxidation.

Biochem/physiol Actions

Although xanthine oxidase is found widely distributed in multiple tissues, it is highly expressed in endothelial and epithelial cells. Its expression was also increased in infected tissues where it was shown to have an anti-microbial effect due to its ability to generate reactive oxygen species. [4]Xanthineoxidase plays an important role in catalyzing the hydroxylation ofhypoxanthine to Xanthine. Later, xanthine is converted to uric acid isexcreted by the kidneys.[1]
Xanthine oxidase is a molybdenum-containing enzyme that is found in the cytosol, and may be strongly inhibited by flavonoids. It plays a vital role in the metabolism of some drugs, as well as purines and pyrimidines. It is also known to be a biological source of reactive oxygen species.

Unit Definition

One unit will convert 1.0 μmole of xanthine to uric acid per min at pH 7.5 at 25 °C. Approx. 50% of the activity is obtained with hypoxanthine as substrate.

Physical form

Suspension in 2.3 M (NH4)2SO4 containing 1 mM sodium salicylate

Analysis Note

Protein determined by biuret

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000299810
0000299810
0000292863
0000292865
0000292864

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João L Serrano et al.
Biomedicines, 9(10) (2021-10-24)
Xanthine oxidase (XO) is the enzyme responsible for the conversion of endogenous purines into uric acid. Therefore, this enzyme has been associated with pathological conditions caused by hyperuricemia, such as the disease commonly known as gout. Barbiturates and their congeners
Role of xanthine oxidoreductase as an antimicrobial agent.
Hannah M Martin et al.
Infection and immunity, 72(9), 4933-4939 (2004-08-24)
Evolution of the anticholinesterase, antioxidant, and anti-inflammatory activity of Epilobium angustifolium L. infusion during in vitro digestion
Szwajgier D, et al.
Journal of functional foods, 85, 104645-104645 (2021)
Szymon Byczkiewicz et al.
Nutrients, 16(11) (2024-06-19)
In the present study, the effect of the addition of quince and collagen type I and III to dessert chocolate on its functional properties was determined. The study evaluated the antioxidant potential of the tested formulations using the FRAP method
M B Betancor et al.
Fish physiology and biochemistry, 41(3), 705-720 (2015-03-10)
In vertebrates, selenium (Se) is an essential micronutrient for vertebrates that is involved in antioxidant protection and thyroid hormone regulation among other roles and functions through its incorporation into proteins, the selenoproteins. Long-chain polyunsaturated fatty acids, such as docosahexaenoic acid
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Questions

  1. Hello, Is there a way to find out how much 2.3M (NH4)2SO4 was used to suspend 10 Units of Xanthine Oxidase? Specifically, what is the ratio of units Xanthine Oxidase (or mg) to volume needed to create this suspension using lyophilized powder? Thank you!

    1 answer

    1. The volume of a given package size varies from lot to lot. The minimum activity of this product is 0.1 units per milligram of protein. The minimum protein concentration is 5.0 mg/mL. As an example, a lot reporting an activity of 0.3 units per milligram protein at a concentration of 13.8 mg protein per milliliter, will have a volume of 2.3 mL for the 10 Unit package size. This is determined by using the following calculation:
      10U / (0.3U/mg) = 30mg / (13.8mg/ml) = 2.3 ml volume

      The lot-specific volume may be determined by data provided in the Certificate of Analysis. Please see the link below to review a sample or lot-specific Certificate:
      https://www.sigmaaldrich.com/product/sigma/x4875#product-documentation

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